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Catalog Products » Other Products » MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), mAb, Rabbit

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), mAb, Rabbit

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

Sensitivity and specificity of MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40) to 6xHis-tag Fusion Protein by Western blot.

Loading: His tagged fusion protein
Primary Antibody:
MonoRabTM Anti-His Tag Antibody (A01857-40) 0.2 μg/ml
Secondary Antibody:
Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

Western blot analysis of immunoprecipitation from cell lysates containing His fusion protein by using MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40)

Loading:
Lane 1: MonoRabTM Anti-His antibody (4 μg) + His tagged fusion protein
Lane 2: Rabbit IgG (4 μg) + His tagged fusion protein
Lane 3: His tagged fusion protein (Input)
Primary Antibody:
THETM His tag antibody, mAb, Mouse (Genscript,A00186) 0.2 μg/ml
Secondary Antibody:
Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

Western blot analysis of cross-reactivity of MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40)
Primary Antibody:
A: MonoRabTM Anti-His Tag Antibody (A01857-40) 1μg/ml
B: Rabbit IgG (Negative control) 1μg/ml
Secondary Antibody:
Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor,926-32211)0.125μg/ml

Description The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRabTM technology to generate high affinity and specific monoclonal rabbit antibodies. GenScript MonoRabᵀᴹ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Host Species Rabbit
Conjugation Unconjugated
Immunogen 6x His synthetic peptide coupled to KLH
Purification Protein A affinity column

Subclass IgG
Clone ID 25B6E11
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It could be customized for bulk order)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity GenScript MonoRabᵀᴹ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit specifically recognizes His tags placed at C-terminal and internal regions of His fusion proteins. This product is not recommended for N-terminal His fusion protein detection.
Storage The lyophilized product remains stable up to 1 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 μg/ml
Western Blot: 0.1-0.2 μg/ml
Immunoprecipitation (IP): 2 μg/ml
Immunofluorescent staining: 1 μg/ml
Flow cytometry (FACS): 1 μg/ml
Other applications: user-optimized

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit
MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

Sensitivity and specificity of MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40) to 6xHis-tag Fusion Protein by Western blot.

Loading: His tagged fusion protein
Primary Antibody:
MonoRabTM Anti-His Tag Antibody (A01857-40) 0.2 μg/ml
Secondary Antibody:
Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit
MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

Western blot analysis of immunoprecipitation from cell lysates containing His fusion protein by using MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40)

Loading:
Lane 1: MonoRabTM Anti-His antibody (4 μg) + His tagged fusion protein
Lane 2: Rabbit IgG (4 μg) + His tagged fusion protein
Lane 3: His tagged fusion protein (Input)
Primary Antibody:
THETM His tag antibody, mAb, Mouse (Genscript,A00186) 0.2 μg/ml
Secondary Antibody:
Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit
MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

Western blot analysis of cross-reactivity of MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40)
Primary Antibody:
A: MonoRabTM Anti-His Tag Antibody (A01857-40) 1μg/ml
B: Rabbit IgG (Negative control) 1μg/ml
Secondary Antibody:
Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor,926-32211)0.125μg/ml




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Cat. No. A01857
Size
Price
40 ug $198.00
24 hour
 
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