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Catalog Products » Antibodies » MonoRab™ Rabbit Monoclonal Antibody » MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit

MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit

MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], MAb, Rabbit

Flow cytometric analysis of CHO/Plasmid cells (Red) and CHO negative control cell (Black) binding with MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit (GenScript, A01871-40)
Antibody working concentration: 5 µg/ml, 2.5x10⁵ cells/reaction

MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], MAb, Rabbit

Immunofluorescence staining of MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit (GenScript, A01871-40) with DYKDDDDK Tag in CHO-K1 cells.
CHO-K1 cells transfected with pcDNA3.1(+) (DYKDDDDK Tag-RFP) (Red) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min.
The cells were then incubated with 1/100 MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit (GenScript, A01871-40) (Green) at 37℃ for 1h.

Description The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRabᵀᴹ technology to generate the high affinity and specificity monoclonal rabbit antibodies. MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit specific to DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of fusion proteins. The antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify DYKDDDDK fusion proteins in bacteria and mammalian cells.
Host Species Rabbit
Conjugation FITC
Immunogen DYKDDDDK synthetic peptide coupled to KLH
Purification Protein A affinity column

Subclass IgG
Clone ID 114F12C8
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% Sodium azide. (It may be customized for bulk orders.)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity GenScript MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit specifically recognizes DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

IF: 1 μg/ml
FACS: 1 μg/ml
Other applications: user-optimized

  • MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], MAb, Rabbit
  • MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], MAb, Rabbit

    Flow cytometric analysis of CHO/Plasmid cells (Red) and CHO negative control cell (Black) binding with MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit (GenScript, A01871-40)
    Antibody working concentration: 5 µg/ml, 2.5x10⁵ cells/reaction

  • MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], MAb, Rabbit
  • MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], MAb, Rabbit

    Immunofluorescence staining of MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit (GenScript, A01871-40) with DYKDDDDK Tag in CHO-K1 cells.
    CHO-K1 cells transfected with pcDNA3.1(+) (DYKDDDDK Tag-RFP) (Red) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min.
    The cells were then incubated with 1/100 MonoRabᵀᴹ DYKDDDDK Tag Antibody [FITC], mAb, Rabbit (GenScript, A01871-40) (Green) at 37℃ for 1h.


Cat. No.
Product Name
Price
A01870-40
MonoRabᵀᴹ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit - 40 µg
$198.00
A01869-40
MonoRabᵀᴹ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit - 40 µg
$198.00
A01868-40
MonoRabᵀᴹ DYKDDDDK Tag Antibody, mAb, Rabbit - 40 µg
$198.00
 


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Cat. No. A01871
Size
Price
40 µg $198.00