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Human LAG3 Antibody (LG.B5), mAb, Mouse

*This product has been discontinued!*
The product is specific for human LAG3. This antibody binds human LAG3 recombinant protein and cell surface-expressing LAG3.
A01876
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Overview
Specificity The product is specific for human LAG3. This antibody binds human LAG3 recombinant protein and cell surface-expressing LAG3.
Host Species Mouse
Immunogen Recombinant human LAG3-Fc (R&D, 2319-L3)
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.1 µg/ml
Flow Cytometry 2-10 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID LG.B5
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Background
Target Background LAG-3 (Lymphocyte activation gene-3), designated CD223, is a 70 kDa type I transmembrane protein that is a member of the immunoglobulin superfamily (IgSF). LAG-3 is expressed on activated CD4+ and CD8+ T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells. LAG-3 on activated CD4+CD25+ Treg cells plays a role in their suppressive activity. LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli. In mice, deletion of LAG-3 and another negative regulator, PD-1, facilitates anti-cancer response but also blocks self-tolerance and increases susceptibility to autoimmune diseases. In humans, antibody-mediated down‑regulation of LAG-3 and PD-1 allows more effective control of chronic malaria, while in NOD (non‑obese diabetic) mice, deletion of LAG-3 alone accelerates diabetes.GenScript Human LAG3 Antibody (LG.B5), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Recombinant human LAG3-Fc (R&D, 2319-L3).
Synonyms Mouse monoclonal to CD223, LAG-3, Ly66, Lymphocyte activation gene 3 protein, lymphocyte-activation gene 3, Protein FDC

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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