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Home » Gene Synthesis & Molecular Biology Services » Human PRTN3 Antibody (15E12D7), mAb, Mouse

Human PRTN3 Antibody (15E12D7), mAb, Mouse

Human PRTN3 Antibody (15E12D7), MAb, Mouse

ELISA binding of Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) with Human PRTN3 recombinant protein.
Coating antigen: PRTN3, 1 µg/ml.
PRTN3 antibody dilution start from 1000 ng/ml,
EC₅₀= 13.17 ng/ml.

Human PRTN3 Antibody (15E12D7), MAb, Mouse

Western Blot validation of Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) with Human PRTN3 recombinant protein.
Lane 1: 50 ng Human PRTN3 recombinant protein
Lane 2: 10 ng Human PRTN3 recombinant protein
Lane 3: 5 ng Human PRTN3 recombinant protein
Primary Antibody:
Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) 1 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

Synonyms Mouse monoclonal to ACPA, AGP7, azurophil granule protein 7, C-ANCA, C-ANCA antigen, CANCA, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, NP-4, NP4, P29, PR-3, PR3, serine proteinase, neutrophil, Wegener autoantigen, Wegener granulomatosis autoantigen
Description PRTN3 (Proteinase 3) is a protein coding gene. Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain. Among its related pathways are Immune System and Cytokine Signaling in Immune system. Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Diseases associated with PRTN3 include Wegener Granulomatosis and Rapidly Progressive Glomerulonephritis.
GenScript Human PRTN3 Antibody (15E12D7), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with recombinant human PRTN3.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen Recombinant Human PRTN3
Purification Protein A affinity column

Subclass IgG2b,κ
Clone ID 15E12D7
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It could be customized for bulk order.)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for human PRTN3. This antibody binds with recombinant PRTN3 protein in ELISA and western blot.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
Western blot: 1 µg/ml
Other applications: user-optimized

  • Human PRTN3 Antibody (15E12D7), MAb, Mouse
  • Human PRTN3 Antibody (15E12D7), MAb, Mouse

    ELISA binding of Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) with Human PRTN3 recombinant protein.
    Coating antigen: PRTN3, 1 µg/ml.
    PRTN3 antibody dilution start from 1000 ng/ml,
    EC₅₀= 13.17 ng/ml.

  • Human PRTN3 Antibody (15E12D7), MAb, Mouse
  • Human PRTN3 Antibody (15E12D7), MAb, Mouse

    Western Blot validation of Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) with Human PRTN3 recombinant protein.
    Lane 1: 50 ng Human PRTN3 recombinant protein
    Lane 2: 10 ng Human PRTN3 recombinant protein
    Lane 3: 5 ng Human PRTN3 recombinant protein
    Primary Antibody:
    Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) 1 μg/ml
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml




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Cat. No. A01883
Size
Price
40 ug $99.00