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Human PRTN3 Antibody (11F1A5), mAb, Mouse

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ELISA binding of Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) with Human PRTN3 recombinant protein.
Coating antigen: PRTN3, 1 µg/ml.
PRTN3 antibody dilutions start from 1000 ng/ml, EC50= 9.667 ng/ml.

Standard curve of PRTN3 Sandwich ELISA. The PRTN3 Sandwich ELISA assay is developed by using Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) and Biotin conjugated Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) as capture and detect antibody, respectively.
From the experimental results, Human PRTN3 Antibody (11F1A5) and Human PRTN3 Antibody (15E12D7) identify different epitopes. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.

Independent validation of Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) and Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) with Human PRTN3 recombinant protein.
Lane 1: 50 ng Human PRTN3 recombinant protein
Lane 2: 10 ng Human PRTN3 recombinant protein
Lane 3: 5 ng Human PRTN3 recombinant protein
Lane 4: 50 ng Human PRTN3 recombinant protein
Lane 5: 10 ng Human PRTN3 recombinant protein
Lane 6: 5 ng Human PRTN3 recombinant protein
Primary Antibody:
Lane 1~3: Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) 1 μg/ml
Lane 4~6: Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) 1 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211)0.125 μg/ml

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Overview

Synonyms	Mouse monoclonal to ACPA, AGP7, azurophil granule protein 7, C-ANCA, C-ANCA antigen, CANCA, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, NP-4, NP4, P29, PR-3, PR3, serine proteinase, neutrophil, Wegener autoantigen, Wegener gra
Description	PRTN3 (Proteinase 3) is a protein coding gene that belongs to the peptidase S1 family, Elastase subfamily. PRTN3 contains a 1 peptidase S1 domain. Among its related pathways are immune system and cytokine signaling in the immune system. PRTN3 degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Diseases associated with PRTN3 include Wegener Granulomatosis and Rapidly Progressive Glomerulonephritis.
Host Species	Mouse
Conjugation	Unconjugated
Immunogen	Recombinant Human PRTN3
Purification	Protein A affinity column

Properties

Subclass	IgG2b,κ
Clone ID	11F1A5
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It may be customized for bulk orders.)
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity	The product is specific for human PRTN3. This antibody binds with recombinant PRTN3 protein in ELISA and Western blot.
Storage	The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
Western blot: 1 µg/ml
Other applications: user-optimized