• English
  • Sign In
  • Contact Us
×
Home » Gene Synthesis & Molecular Biology Services » Human PRTN3 Antibody (11F1A5), mAb, Mouse

Human PRTN3 Antibody (11F1A5), mAb, Mouse

Human PRTN3 Antibody (11F1A5), MAb, Mouse

ELISA binding of Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) with Human PRTN3 recombinant protein.
Coating antigen: PRTN3, 1 µg/ml.
PRTN3 antibody dilutions start from 1000 ng/ml, EC50= 9.667 ng/ml.

Human PRTN3 Antibody (11F1A5), MAb, Mouse

Standard curve of PRTN3 Sandwich ELISA. The PRTN3 Sandwich ELISA assay is developed by using Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) and Biotin conjugated Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) as capture and detect antibody, respectively.
From the experimental results, Human PRTN3 Antibody (11F1A5) and Human PRTN3 Antibody (15E12D7) identify different epitopes. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.

Human PRTN3 Antibody (11F1A5), MAb, Mouse

Independent validation of Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) and Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) with Human PRTN3 recombinant protein.
Lane 1: 50 ng Human PRTN3 recombinant protein
Lane 2: 10 ng Human PRTN3 recombinant protein
Lane 3: 5 ng Human PRTN3 recombinant protein
Lane 4: 50 ng Human PRTN3 recombinant protein
Lane 5: 10 ng Human PRTN3 recombinant protein
Lane 6: 5 ng Human PRTN3 recombinant protein
Primary Antibody:
Lane 1~3: Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) 1 μg/ml
Lane 4~6: Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) 1 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211)0.125 μg/ml

Synonyms Mouse monoclonal to ACPA, AGP7, azurophil granule protein 7, C-ANCA, C-ANCA antigen, CANCA, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, NP-4, NP4, P29, PR-3, PR3, serine proteinase, neutrophil, Wegener autoantigen, Wegener gra
Description PRTN3 (Proteinase 3) is a protein coding gene that belongs to the peptidase S1 family, Elastase subfamily. PRTN3 contains a 1 peptidase S1 domain. Among its related pathways are immune system and cytokine signaling in the immune system. PRTN3 degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Diseases associated with PRTN3 include Wegener Granulomatosis and Rapidly Progressive Glomerulonephritis.
Host Species Mouse
Conjugation Unconjugated
Immunogen Recombinant Human PRTN3
Purification Protein A affinity column

Subclass IgG2b,κ
Clone ID 11F1A5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It may be customized for bulk orders.)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for human PRTN3. This antibody binds with recombinant PRTN3 protein in ELISA and Western blot.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
Western blot: 1 µg/ml
Other applications: user-optimized

  • Human PRTN3 Antibody (11F1A5), MAb, Mouse
  • Human PRTN3 Antibody (11F1A5), MAb, Mouse

    ELISA binding of Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) with Human PRTN3 recombinant protein.
    Coating antigen: PRTN3, 1 µg/ml.
    PRTN3 antibody dilutions start from 1000 ng/ml, EC50= 9.667 ng/ml.

  • Human PRTN3 Antibody (11F1A5), MAb, Mouse
  • Human PRTN3 Antibody (11F1A5), MAb, Mouse

    Standard curve of PRTN3 Sandwich ELISA. The PRTN3 Sandwich ELISA assay is developed by using Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) and Biotin conjugated Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) as capture and detect antibody, respectively.
    From the experimental results, Human PRTN3 Antibody (11F1A5) and Human PRTN3 Antibody (15E12D7) identify different epitopes. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.

  • Human PRTN3 Antibody (11F1A5), MAb, Mouse
  • Human PRTN3 Antibody (11F1A5), MAb, Mouse

    Independent validation of Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) and Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) with Human PRTN3 recombinant protein.
    Lane 1: 50 ng Human PRTN3 recombinant protein
    Lane 2: 10 ng Human PRTN3 recombinant protein
    Lane 3: 5 ng Human PRTN3 recombinant protein
    Lane 4: 50 ng Human PRTN3 recombinant protein
    Lane 5: 10 ng Human PRTN3 recombinant protein
    Lane 6: 5 ng Human PRTN3 recombinant protein
    Primary Antibody:
    Lane 1~3: Human PRTN3 Antibody (11F1A5) (GenScript, A01884-40) 1 μg/ml
    Lane 4~6: Human PRTN3 Antibody (15E12D7) (GenScript, A01883-40) 1 μg/ml
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211)0.125 μg/ml




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. A01884
Size
Price
40 µg $99.00