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Home » Gene Synthesis & Molecular Biology Services » Mouse B4galt1 Antibody (3C9), mAb, Mouse

Mouse B4galt1 Antibody (3C9), mAb, Mouse

Mouse B4galt1 Antibody (3C9), MAb, Mouse

ELISA binding of Mouse B4galt1 Antibody (3C9) (GenScript, A01889-40) with Mouse B4galt1 recombinant protein.
Coating antigen: B4galt1, 1 µg/ml.
B4galt1 antibody dilutions start from 1000 ng/ml, EC50= 29.73 ng/ml.

Mouse B4galt1 Antibody (3C9), MAb, Mouse

Standard curve of B4galt1 Sandwich ELISA. The B4galt1 Sandwich ELISA assay is developed by using Mouse B4galt1 Antibody (11H3) (GenScript, A01890-40) and Biotin conjugated Mouse B4galt1 Antibody (3C9) (GenScript, A01889-40) as capture and detect antibody, respectively.
From the experimental results, Mouse B4galt1 Antibody (11H3) and Mouse B4galt1 Antibody (3C9) identify different epitopes. The sensitivity is 1-2 ng/ml and the detection range is 0-100 ng/ml.

Mouse B4galt1 Antibody (3C9), MAb, Mouse

Independent validation of Mouse B4galt1 Antibody (3C9) (GenScript, A01889-40) and Mouse B4galt1 Antibody (11H3) (GenScript, A01890-40) with Mouse B4galt1 recombinant protein.
Lane 1: 50 ng Mouse B4galt1 recombinant protein
Lane 2: 25 ng Mouse B4galt1 recombinant protein
Lane 3: 10 ng Mouse B4galt1 recombinant protein
Lane 4: 50 ng Mouse B4galt1 recombinant protein
Lane 5: 25 ng Mouse B4galt1 recombinant protein
Lane 6: 10 ng Mouse B4galt1 recombinant protein
Primary Antibody:
Lane 1~3: Mouse B4galt1 Antibody (3C9) (GenScript, A01889-40) 1 μg/ml
Lane 4~6: Mouse B4galt1 Antibody (11H3) (GenScript, A01890-40) 1 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

Synonyms Mouse monoclonal to Beta-1,4-galactosyltransferase 1, Beta-1,4-GalTase 1, Beta4Gal-T1, b4Gal-T1, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
Description B4GALT1 (Beta-1,4-Galactosyltransferase 1) is a protein coding gene. Among its related pathways are transport to the Golgi and subsequent modification and metabolism. B4galt1 is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose. The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
Host Species Mouse
Conjugation Unconjugated
Immunogen Recombinant Mouse B4galt1
Purification Protein A affinity column

Subclass IgG2b,κ
Clone ID 3C9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It may be customized for bulk orders.)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for mouse B4galt1. This antibody binds with recombinant B4galt1 protein in ELISA and western blot.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.05-0.1 µg/ml
Western blot: 1 µg/ml
Other applications: user-optimized

  • Mouse B4galt1 Antibody (3C9), MAb, Mouse
  • Mouse B4galt1 Antibody (3C9), MAb, Mouse

    ELISA binding of Mouse B4galt1 Antibody (3C9) (GenScript, A01889-40) with Mouse B4galt1 recombinant protein.
    Coating antigen: B4galt1, 1 µg/ml.
    B4galt1 antibody dilutions start from 1000 ng/ml, EC50= 29.73 ng/ml.

  • Mouse B4galt1 Antibody (3C9), MAb, Mouse
  • Mouse B4galt1 Antibody (3C9), MAb, Mouse

    Standard curve of B4galt1 Sandwich ELISA. The B4galt1 Sandwich ELISA assay is developed by using Mouse B4galt1 Antibody (11H3) (GenScript, A01890-40) and Biotin conjugated Mouse B4galt1 Antibody (3C9) (GenScript, A01889-40) as capture and detect antibody, respectively.
    From the experimental results, Mouse B4galt1 Antibody (11H3) and Mouse B4galt1 Antibody (3C9) identify different epitopes. The sensitivity is 1-2 ng/ml and the detection range is 0-100 ng/ml.

  • Mouse B4galt1 Antibody (3C9), MAb, Mouse
  • Mouse B4galt1 Antibody (3C9), MAb, Mouse

    Independent validation of Mouse B4galt1 Antibody (3C9) (GenScript, A01889-40) and Mouse B4galt1 Antibody (11H3) (GenScript, A01890-40) with Mouse B4galt1 recombinant protein.
    Lane 1: 50 ng Mouse B4galt1 recombinant protein
    Lane 2: 25 ng Mouse B4galt1 recombinant protein
    Lane 3: 10 ng Mouse B4galt1 recombinant protein
    Lane 4: 50 ng Mouse B4galt1 recombinant protein
    Lane 5: 25 ng Mouse B4galt1 recombinant protein
    Lane 6: 10 ng Mouse B4galt1 recombinant protein
    Primary Antibody:
    Lane 1~3: Mouse B4galt1 Antibody (3C9) (GenScript, A01889-40) 1 μg/ml
    Lane 4~6: Mouse B4galt1 Antibody (11H3) (GenScript, A01890-40) 1 μg/ml
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml


Cat. No.
Product Name
Price
A01890-40
Mouse B4galt1 Antibody (11H3), mAb, Mouse - 40 µg
$99.00
 


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Cat. No. A01889
Size
Price
40 µg $99.00