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Home » Gene Synthesis & Molecular Biology Services » Human C-reactive protein Antibody (8B11) [Biotin], mAb, Mouse

Human C-reactive protein Antibody (8B11) [Biotin], mAb, Mouse

Human C-reactive Protein Antibody (8B11) [Biotin], MAb, Mouse

ELISA binding of Human CRP Antibody (8B11) [Biotin], mAb, Mouse (GenScript, A01920-40) with Human CRP.
Coating antigen: Human CRP, 1 µg/ml,
Human CRP antibody dilution start from 2000 ng/ml, EC₅₀=15.32 ng/ml.

Human C-reactive Protein Antibody (8B11) [Biotin], MAb, Mouse

Western blot analysis of Human CRP Antibody (8B11) [Biotin], mAb, Mouse (GenScript, A01920-40) with Human CRP.
Loading:
Lane 1 : Human CRP (100 ng)
Lane 2 : Human CRP (50 ng)
Lane 3 : Human CRP (10 ng)
Lane 4 : Human CRP (5 ng)
Lane 5 : Human CRP (1 ng)
Primary Antibody:
Human CRP Antibody (8B11) [Biotin], mAb, Mouse (GenScript, A01920-40) 1μg/ml
Secondary Antibody:
Streptavidin-HRP (Genscript, M00091, 15L000972) 0.2μg/ml

Synonyms Mouse monoclonal to CRP/PTX1
Description C-reactive protein (CRP) is an annular, pentameric protein found in blood plasma, in which the levels rise in response to inflammation. It is an acute-phase protein synthesized by the liver that increases following interleukin-6 secretion by macrophages and T cells. CRP binds to the phosphocholine expressed on the surface of dead or dying cells and some bacteria. This activates the complement system, promoting phagocytosis by macrophages, which clears necrotic and apoptotic cells and bacteria.
Host Species Mouse
Conjugation Biotin
Immunogen Human CRP
Purification Protein A affinity column

Subclass IgG1,κ
Clone ID 8B11
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. (It may be customized for bulk orders.)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for human CRP. This antibody binds with human CRP in ELISA and Western blot assay. The antibody is recommended as a detection antibody in a Sandwich ELISA assay with capture antibody GenScript, A01919-40, Human C-reactive protein Antibody (39E5), mAb, Mouse.
Storage The lyophilized product remains stable up to 1 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA detection: 0.02-0.2 µg/ml
Western blot: 1 µg/ml
Other applications: user-optimized

  • Human C-reactive Protein Antibody (8B11) [Biotin], MAb, Mouse
  • Human C-reactive Protein Antibody (8B11) [Biotin], MAb, Mouse

    ELISA binding of Human CRP Antibody (8B11) [Biotin], mAb, Mouse (GenScript, A01920-40) with Human CRP.
    Coating antigen: Human CRP, 1 µg/ml,
    Human CRP antibody dilution start from 2000 ng/ml, EC₅₀=15.32 ng/ml.

  • Human C-reactive Protein Antibody (8B11) [Biotin], MAb, Mouse
  • Human C-reactive Protein Antibody (8B11) [Biotin], MAb, Mouse

    Western blot analysis of Human CRP Antibody (8B11) [Biotin], mAb, Mouse (GenScript, A01920-40) with Human CRP.
    Loading:
    Lane 1 : Human CRP (100 ng)
    Lane 2 : Human CRP (50 ng)
    Lane 3 : Human CRP (10 ng)
    Lane 4 : Human CRP (5 ng)
    Lane 5 : Human CRP (1 ng)
    Primary Antibody:
    Human CRP Antibody (8B11) [Biotin], mAb, Mouse (GenScript, A01920-40) 1μg/ml
    Secondary Antibody:
    Streptavidin-HRP (Genscript, M00091, 15L000972) 0.2μg/ml


Cat. No.
Product Name
Price
A01919-40
Human C-reactive protein Antibody (39E5), mAb, Mouse - 40 µg
$99.00
 



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Cat. No. A01920
Size
Price
40 µg $99.00