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Home » Gene Synthesis & Molecular Biology Services » Mouse Wdtc1 Antibody (1B12), mAb, Mouse

Mouse Wdtc1 Antibody (1B12), mAb, Mouse

Mouse Wdtc1 Antibody (1B12), MAb, Mouse

Standard curve of Wdtc1 Sandwich ELISA. The Wdtc1 Sandwich ELISA assay is developed by using Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) and Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40) as capture and detection antibody, respectively. These two antibodies recognize different epitopes.
In this ELISA assay, Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40) was labeled with Biotin. GenScript can provide customized conjugation service for this product per customer's request.
The sensitivity is <30 ng/ml and the detection range is 0-1000 ng/ml.

Mouse Wdtc1 Antibody (1B12), MAb, Mouse

Western Blot of Mouse Wdtc1 recombinant protein with two independent antibodies: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) and Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40). The correlated pattern indicates the high specificity of these two antibodies.
Lane 1: 50 ng Mouse Wdtc1 recombinant protein
Lane 2: 25 ng Mouse Wdtc1 recombinant protein
Lane 3: 10 ng Mouse Wdtc1 recombinant protein
Lane 4: 50 ng Mouse Wdtc1 recombinant protein
Lane 5: 25 ng Mouse Wdtc1 recombinant protein
Lane 6: 10 ng Mouse Wdtc1 recombinant protein
Primary Antibody:
Lane 1~3: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 1 μg/ml
Lane 4~6: Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40) 1 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

Mouse Wdtc1 Antibody (1B12), MAb, Mouse

Western Blot of Wild-type and Wdtc1 knockout HepG2 cell lysates with two independent antibodies: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) and Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40). The correlated pattern minimizes the likelihood of off-target binding to unrelated protein.
Lane 1: 50 μg Wild-type HepG2 cell Lysate
Lane 2: 50 μg Wdtc1 knockout HepG2 cell Lysate
Lane 3: 50 μg Wdtc1 knockout HepG2 cell Lysate
Lane 4: 50 μg Wdtc1 knockout HepG2 cell Lysate
Lane 5: 50 μg Wild-type HepG2 cell Lysate
Lane 6: 50 μg Wdtc1 knockout HepG2 cell Lysate
Lane 7: 50 μg Wdtc1 knockout HepG2 cell Lysate
Lane 8: 50 μg Wdtc1 knockout HepG2 cell Lysate
Primary Antibody:
Lane 1~4: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 1 μg/ml
Lane 5~8: Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40) 1 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

Mouse Wdtc1 Antibody (1B12), MAb, Mouse

Western Blot of Wild-type and Wdtc1 knockout HepG2 cell lysates with Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40). The different concentration of cell lysates indicates the high specificity and sensitivity of the antibody.
Lane 1: 50 μg Wild-type HepG2 cell Lysate
Lane 2: 25 μg Wild-type HepG2 cell Lysate
Lane 3: 10 μg Wild-type HepG2 cell Lysate
Lane 4: 50 μg Wdtc1 knockout HepG2 cell Lysate
Primary Antibody:
Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 1 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

Mouse Wdtc1 Antibody (1B12), MAb, Mouse

Western Blot of Wild-type and Wdtc1 knockout HepG2 cell lysates with Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40). The different concentration of antibodies indicates the high specificity and sensitivity of the antibody.
Lane 1: 50 μg Wild-type HepG2 cell Lysate
Lane 2: 50 μg Wdtc1 knockout HepG2 cell Lysate
Lane 3: 50 μg Wild-type HepG2 cell Lysate
Lane 4: 50 μg Wdtc1 knockout HepG2 cell Lysate
Lane 5: 50 μg Wild-type HepG2 cell Lysate
Lane 6: 50 μg Wdtc1 knockout HepG2 cell Lysate
Primary Antibody:
Lane 1~2: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 2.5 μg/ml
Lane 3~4: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 1 μg/ml
Lane 5~6: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 0.5 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

Description WDTC1 (WD and tetratricopeptide repeats protein 1) may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Pathways related to this gene include histone binding, enzyme inhibitor activity, protein ubiquitination and protein modification. Diseases associated with WDTC1 include Hermansky-Pudlak Syndrome 2 and Commensal Bacterial Infectious Disease.
Host Species Mouse
Conjugation Unconjugated
Immunogen Recombinant Mouse Wdtc1
Purification Protein A affinity column

Subclass IgG2a,λ
Clone ID 1B12
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, contains 0.02% sodium azide. (Might be eligible for customized bulk orders.)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is KO Validated by Western Blot. This antibody binds with recombinant mouse Wdtc1 protein and Endogenous human Wdtc1 in western blot.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA detection: 0.5-1 µg/ml
Western blot: 0.5-5 µg/ml
Other applications: user-optimized

  • Mouse Wdtc1 Antibody (1B12), MAb, Mouse
  • Mouse Wdtc1 Antibody (1B12), MAb, Mouse

    Standard curve of Wdtc1 Sandwich ELISA. The Wdtc1 Sandwich ELISA assay is developed by using Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) and Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40) as capture and detection antibody, respectively. These two antibodies recognize different epitopes.
    In this ELISA assay, Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40) was labeled with Biotin. GenScript can provide customized conjugation service for this product per customer's request.
    The sensitivity is <30 ng/ml and the detection range is 0-1000 ng/ml.

  • Mouse Wdtc1 Antibody (1B12), MAb, Mouse
  • Mouse Wdtc1 Antibody (1B12), MAb, Mouse

    Western Blot of Mouse Wdtc1 recombinant protein with two independent antibodies: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) and Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40). The correlated pattern indicates the high specificity of these two antibodies.
    Lane 1: 50 ng Mouse Wdtc1 recombinant protein
    Lane 2: 25 ng Mouse Wdtc1 recombinant protein
    Lane 3: 10 ng Mouse Wdtc1 recombinant protein
    Lane 4: 50 ng Mouse Wdtc1 recombinant protein
    Lane 5: 25 ng Mouse Wdtc1 recombinant protein
    Lane 6: 10 ng Mouse Wdtc1 recombinant protein
    Primary Antibody:
    Lane 1~3: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 1 μg/ml
    Lane 4~6: Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40) 1 μg/ml
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

  • Mouse Wdtc1 Antibody (1B12), MAb, Mouse
  • Mouse Wdtc1 Antibody (1B12), MAb, Mouse

    Western Blot of Wild-type and Wdtc1 knockout HepG2 cell lysates with two independent antibodies: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) and Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40). The correlated pattern minimizes the likelihood of off-target binding to unrelated protein.
    Lane 1: 50 μg Wild-type HepG2 cell Lysate
    Lane 2: 50 μg Wdtc1 knockout HepG2 cell Lysate
    Lane 3: 50 μg Wdtc1 knockout HepG2 cell Lysate
    Lane 4: 50 μg Wdtc1 knockout HepG2 cell Lysate
    Lane 5: 50 μg Wild-type HepG2 cell Lysate
    Lane 6: 50 μg Wdtc1 knockout HepG2 cell Lysate
    Lane 7: 50 μg Wdtc1 knockout HepG2 cell Lysate
    Lane 8: 50 μg Wdtc1 knockout HepG2 cell Lysate
    Primary Antibody:
    Lane 1~4: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 1 μg/ml
    Lane 5~8: Mouse Wdtc1 Antibody (8C8) (GenScript, A01925-40) 1 μg/ml
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

  • Mouse Wdtc1 Antibody (1B12), MAb, Mouse
  • Mouse Wdtc1 Antibody (1B12), MAb, Mouse

    Western Blot of Wild-type and Wdtc1 knockout HepG2 cell lysates with Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40). The different concentration of cell lysates indicates the high specificity and sensitivity of the antibody.
    Lane 1: 50 μg Wild-type HepG2 cell Lysate
    Lane 2: 25 μg Wild-type HepG2 cell Lysate
    Lane 3: 10 μg Wild-type HepG2 cell Lysate
    Lane 4: 50 μg Wdtc1 knockout HepG2 cell Lysate
    Primary Antibody:
    Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 1 μg/ml
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

  • Mouse Wdtc1 Antibody (1B12), MAb, Mouse
  • Mouse Wdtc1 Antibody (1B12), MAb, Mouse

    Western Blot of Wild-type and Wdtc1 knockout HepG2 cell lysates with Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40). The different concentration of antibodies indicates the high specificity and sensitivity of the antibody.
    Lane 1: 50 μg Wild-type HepG2 cell Lysate
    Lane 2: 50 μg Wdtc1 knockout HepG2 cell Lysate
    Lane 3: 50 μg Wild-type HepG2 cell Lysate
    Lane 4: 50 μg Wdtc1 knockout HepG2 cell Lysate
    Lane 5: 50 μg Wild-type HepG2 cell Lysate
    Lane 6: 50 μg Wdtc1 knockout HepG2 cell Lysate
    Primary Antibody:
    Lane 1~2: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 2.5 μg/ml
    Lane 3~4: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 1 μg/ml
    Lane 5~6: Mouse Wdtc1 Antibody (1B12) (GenScript, A01924-40) 0.5 μg/ml
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml




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Cat. No. A01924
Size
Price
40 µg $198.00