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Home » Gene Synthesis & Molecular Biology Services » Staphylocoagulase Antibody(10B3),mAb,Mouse

Staphylocoagulase Antibody(10B3),mAb,Mouse

Staphylocoagulase Antibody(10B3),mAb,Mouse

ELISA binding of Staphylocoagulase Antibody (10B3) (GenScript, A01926-40) with Staphylocoagulase recombinant protein.
Coating antigen: Staphylocoagulase, 1 µg/ml.
Staphylocoagulase antibody dilution start from 2500 ng/ml,
EC50= 27.72 ng/ml.

Staphylocoagulase Antibody(10B3),mAb,Mouse

Standard curve of Staphylocoagulase Sandwich ELISA. The Staphylocoagulase Sandwich ELISA assay is developed by using Staphylocoagulase Antibody (14A10) (GenScript, A01927-40) and Staphylocoagulase Antibody (10B3) (GenScript, A01926-40) as the capture and detection antibodies, respectively. These two antibodies recognize different epitopes.
In this ELISA assay, Staphylocoagulase Antibody (10B3) (GenScript, A01926-40) was labeled with Biotin.GenScript can provide customized conjugation services for this product per customer's request.
The sensitivity is <1 ng/ml and the detection range is 0-30 ng/ml.

Staphylocoagulase Antibody(10B3),mAb,Mouse

Western Blot of Staphylocoagulase recombinant protein with two independent antibodies: Staphylocoagulase Antibody (10B3) (GenScript, A01926-40) and Staphylocoagulase Antibody (14A10) (GenScript, A01927-40). The correlated pattern indicates the high specificity of these two antibodies.
Lane 1: 100 ng Staphylocoagulase recombinant protein
Lane 2: 50 ng Staphylocoagulase recombinant protein
Lane 3: 10 ng Staphylocoagulase recombinant protein
Lane 4: 5 ng Staphylocoagulase recombinant protein
Lane 5: 100 ng Staphylocoagulase recombinant protein
Lane 6: 50 ng Staphylocoagulase recombinant protein
Lane 6: 10 ng Staphylocoagulase recombinant protein
Lane 6: 5 ng Staphylocoagulase recombinant protein
Primary Antibody:
Lane 1~3: Staphylocoagulase Antibody (10B3) (GenScript, A01926-40) 1 μg/ml
Lane 4~6: Staphylocoagulase Antibody (14A10) (GenScript, A01927-40) 1 μg/ml
Secondary Antibody:
Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml

Description Staphylocoagulase (SC) is a protein secreted by most strains of Staphylococcus aureus, a potent human pathogenic bacterium. SC causes blood clotting by direct activation of the thrombin precursor, prothrombin (ProT). This occurs through a non-proteolytic, cofactor-induced mechanism of activation called “molecular sexuality”. Staphylocoagulase is a potent nonproteolytic prothrombin (ProT) activator and the prototype of a newly established zymogen activator and adhesion protein family. SC-bound ProT efficiently clots fibrinogen, thus bypassing the physiological blood coagulation pathway.
Host Species Mouse
Conjugation Unconjugated
Immunogen Recombinant Staphylocoagulase
Purification Protein A affinity column

Subclass IgG2b,k
Clone ID 10B3
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It may be customized for bulk orders.)
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for Staphylocoagulase. This antibody binds with recombinant Staphylocoagulase protein in ELISA and western blot.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.05-1 µg/ml
Western blot: 1 µg/ml
Other applications: user-optimized

  • Staphylocoagulase Antibody(10B3),mAb,Mouse
  • Staphylocoagulase Antibody(10B3),mAb,Mouse

    ELISA binding of Staphylocoagulase Antibody (10B3) (GenScript, A01926-40) with Staphylocoagulase recombinant protein.
    Coating antigen: Staphylocoagulase, 1 µg/ml.
    Staphylocoagulase antibody dilution start from 2500 ng/ml,
    EC50= 27.72 ng/ml.

  • Staphylocoagulase Antibody(10B3),mAb,Mouse
  • Staphylocoagulase Antibody(10B3),mAb,Mouse

    Standard curve of Staphylocoagulase Sandwich ELISA. The Staphylocoagulase Sandwich ELISA assay is developed by using Staphylocoagulase Antibody (14A10) (GenScript, A01927-40) and Staphylocoagulase Antibody (10B3) (GenScript, A01926-40) as the capture and detection antibodies, respectively. These two antibodies recognize different epitopes.
    In this ELISA assay, Staphylocoagulase Antibody (10B3) (GenScript, A01926-40) was labeled with Biotin.GenScript can provide customized conjugation services for this product per customer's request.
    The sensitivity is <1 ng/ml and the detection range is 0-30 ng/ml.

  • Staphylocoagulase Antibody(10B3),mAb,Mouse
  • Staphylocoagulase Antibody(10B3),mAb,Mouse

    Western Blot of Staphylocoagulase recombinant protein with two independent antibodies: Staphylocoagulase Antibody (10B3) (GenScript, A01926-40) and Staphylocoagulase Antibody (14A10) (GenScript, A01927-40). The correlated pattern indicates the high specificity of these two antibodies.
    Lane 1: 100 ng Staphylocoagulase recombinant protein
    Lane 2: 50 ng Staphylocoagulase recombinant protein
    Lane 3: 10 ng Staphylocoagulase recombinant protein
    Lane 4: 5 ng Staphylocoagulase recombinant protein
    Lane 5: 100 ng Staphylocoagulase recombinant protein
    Lane 6: 50 ng Staphylocoagulase recombinant protein
    Lane 6: 10 ng Staphylocoagulase recombinant protein
    Lane 6: 5 ng Staphylocoagulase recombinant protein
    Primary Antibody:
    Lane 1~3: Staphylocoagulase Antibody (10B3) (GenScript, A01926-40) 1 μg/ml
    Lane 4~6: Staphylocoagulase Antibody (14A10) (GenScript, A01927-40) 1 μg/ml
    Secondary Antibody:
    Goat anti-Mouse IgG (H&L) [IRDye⁸⁰⁰] (Licor,926-32211) 0.125 μg/ml


Cat. No.
Product Name
Price
A01927-40
Staphylocoagulase Antibody(14A10), mAb, Mouse - 40 µg
$99.00
 


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Cat. No. A01926
Size
Price
40 µg $99.00