|Description||HVEM, also known as CD270, is a type 1 transmembrane protein. It is a member of the TNFR superfamily with a molecular weight of 30kDa. HVEM is expressed on hematopoietic cells such as T cells, B cells, dendritic cells, monocytes, neutrophils and NK cells. It participates in regulating lymphocyte activation and homeostasis in immune responses. HVEM activates NF-kB through the TNF-related cytokine LIGHT during T cell activation.|
|Immunogen||Recombinant HVEM protein|
|Purification||Protein A affinity column|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.|
|Specificity||The product is specific for human HVEM|
|Storage||The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.|
|Note||GenScript can customize this product per customer's request including product size, buffer components, etc.|
|Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.05 µg/ml
FACS detection: 1- 5 µg/ml
Other applications: user-optimized
Flow cytometric analysis of peripheral blood mononuclear cell (PBMCs) binding with Human HVEM Antibody(10C6)，mAb，Mouse (GenScript, A01959; blue curve) or binding with an isotype control antibody (red curve), or binding with PBS solution as a negative control (black curve)
Antibody working concentration: 1 µg/ml, 2.5x10⁵ cells/reaction
The signal was developed with R-PE conjugated anti-mouse IgG
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