|Description||CD27, also known as S152 and T14, is a member of the tumor necrosis factor receptor family. It is expressed on memory B cells, a fraction of plasma cells, naive and memory T cells and NK cells. CD27 binds to ligand CD70, and plays an important role in regulating B-cell activation and immunoglobulin synthesis. It is considered as a useful marker to distinguish certain T and B cell subsets from each other.|
|Immunogen||Recombinant CD27 protein|
|Purification||Protein A affinity column|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.|
|Specificity||The product is specific for human CD27|
|Storage||The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.|
|Note||GenScript can customize this product per customer's request including product size, buffer components, etc.|
|Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.05 µg/ml
FACS detection: 1- 5 µg/ml
Other applications: user-optimized
Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) binding with Human CD27 Antibody (GenScript, A01964-40; blue curve) or binding with an isotype control antibody (red curve), or binding with PBS solution as a negative control (black curve)
Antibody working concentration: 1 µg/ml, 2.5x10⁵ cells/reaction
The signal was developed with R-PE conjugated anti-mouse IgG
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