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Home » Gene Synthesis & Molecular Biology Services » Human CD80 Antibody (17F3), mAb, Mouse

Human CD80 Antibody (17F3), mAb, Mouse

Human CD80 Antibody (17F3), MAb, Mouse

Flow cytometric analysis of CHO-K1/CD80 stable cell line (GenScript,M00545,Red) and CHO negative control cell (Black) binding with Human CD80 Antibody (17F3), mAb, Mouse (GenScript, A01973-40).Antibody working concentration: 5 µg/ml, 2.5x105 cells/reaction.
The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG

Human CD80 Antibody (17F3), MAb, Mouse

Flow cytometric analysis shows that Human CD80 Antibody (17F3), mAb, Mouse (GenScript, A01973-40) blocks CD28 Fc Chimera, Human (GenScript, Z03413) binding with CHO-K1/CD80 stable cell line (GenScript, M00545, Red) and its binding without the ligand serves as a control (Black).Ligand: CD28 Fc Chimera, Human, 2.5 µg/ml.
Antibody working concentration: 10 µg/ml, 2.5x105 cells/reaction.
The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG

Synonyms Mouse monoclonal to Human B7-1/BB1/CD28LG/CD28LG1/LAB7
Description CD80, also known as B7-1, is a single chain type I glycoprotein which belongs to the immunoglobulin superfamily. It is expressed on the surface of activated B and T cells, dendritic cells, and macrophages. It plays an important role in regulation of T cell activation. The interaction of CD80 with CD28 and CD152 (CTLA-4) induces and regulates immune responses.
Human CD80 Antibody (17F3), mAb, Mouse is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with CD80.
Host Species Mouse
Conjugation Unconjugated
Immunogen Recombinant CD80 protein
Purification Protein A affinity column

Subclass IgG1
Clone ID 17F3
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The product is specific for human CD80
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

FACS Blocking: 5- 10 µg/ml
ELISA detection: 0.01-0.05 µg/ml
FACS detection: 1- 5 µg/ml
Other applications: user-optimized

  • Human CD80 Antibody (17F3), MAb, Mouse
  • Human CD80 Antibody (17F3), MAb, Mouse

    Flow cytometric analysis of CHO-K1/CD80 stable cell line (GenScript,M00545,Red) and CHO negative control cell (Black) binding with Human CD80 Antibody (17F3), mAb, Mouse (GenScript, A01973-40).Antibody working concentration: 5 µg/ml, 2.5x105 cells/reaction.
    The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG

  • Human CD80 Antibody (17F3), MAb, Mouse
  • Human CD80 Antibody (17F3), MAb, Mouse

    Flow cytometric analysis shows that Human CD80 Antibody (17F3), mAb, Mouse (GenScript, A01973-40) blocks CD28 Fc Chimera, Human (GenScript, Z03413) binding with CHO-K1/CD80 stable cell line (GenScript, M00545, Red) and its binding without the ligand serves as a control (Black).Ligand: CD28 Fc Chimera, Human, 2.5 µg/ml.
    Antibody working concentration: 10 µg/ml, 2.5x105 cells/reaction.
    The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG


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Cat. No. A01973
Size
Price
40 µg $99.00