|Description||CD86, known as B7-2, B70, and Ly-58, is a member of immunoglobulin superfamily. It is expressed on the surface of B lymphocytes, T lymphocytes, dendritic cells and astrocytes. As a ligand for CD28 and CD152 (CTLA-4), CD86 plays a critical role in T cell-B cell interactions. The interaction of CD86 with CD28 can transduce co-stimulatory signals for T cell proliferation and cytokine production. CD86 is also involved in NK cell-mediated cytotoxicity. Human CD86 Antibody (2B11), mAb, Mouse is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with CD86.|
|Immunogen||Recombinant CD86 protein|
|Purification||Protein A affinity column|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.|
|Specificity||The product is specific for human CD86|
|Storage||The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.|
|Note||GenScript can customize this product per customer's request including product size, buffer components, etc.|
|Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.05 µg/ml
FACS detection: 1- 5 µg/ml
Other applications: user-optimized
Flow cytometric analysis of CHO-K1/CD86 stable cell line (Red) and CHO negative control cell (Black) binding with Human CD86 Antibody (2B11), mAb, Mouse (GenScript, A01982-40)Antibody working concentration: 5 µg/ml, 2.5x105 cells/reactionThe signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG
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