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Home » Gene Synthesis & Molecular Biology Services » MonoRabᵀᴹ HA tag Antibody(109B2)[Biotin], mAb, Rabbit

MonoRabᵀᴹ HA tag Antibody(109B2)[Biotin], mAb, Rabbit

Description The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRabᵀᴹ technology to generate high affinity and specific monoclonal rabbit antibodies. The HA tag is composed of several amino acids containing YPYDVPDYA and does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins.
GenScript MonoRabᵀᴹ HA tag Antibody (109B2) [Biotin], mAb, Rabbit is the biotin conjugated form of MonoRabᵀᴹ HA tag Antibody (109B2), mAb, Rabbit. This antibody is suitable for detecting the expression level of HA tag fusion proteins.
Host Species Rabbit
Conjugation Biotin
Immunogen A synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). conjugated to KLH
Purification Protein A affinity column

Subclass IgG
Clone ID 109B2
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, contains 1% BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity MonoRab™ HA tag Antibody recognizes HA tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.
Storage The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 μg/ml
Western Blot: 0.2-1 μg/ml
Other applications: user-optimized

  • MonoRabᵀᴹ HA Tag Antibody(109B2)[Biotin], MAb, Rabbit
  • MonoRabᵀᴹ HA Tag Antibody(109B2)[Biotin], MAb, Rabbit

    Western blot analysis of MonoRab™ HA tag Antibody (109B2) [Biotin], mAb, Rabbit with HA-tag fusion proteins.
    Loading:
    Lane 1 : 25 ng N-terminal HA-tagged fusion protein
    Lane 2 : 10 ng N-terminal HA-tagged fusion protein
    Lane 3 : 5 ng N-terminal HA-tagged fusion protein
    Lane 4 : 1 ng N-terminal HA-tagged fusion protein
    Lane 5: 25 ng C-terminal HA-tagged fusion protein
    Lane 6: 10 ng C-terminal HA-tagged fusion protein
    Lane 7: 5 ng C-terminal HA-tagged fusion protein
    Lane 8: 1 ng C-terminal HA-tagged fusion protein
    Primary Antibody:
    MonoRabᵀᴹ HA tag Antibody (109B2)[Biotin], mAb, Rabbit (A02002-40) 1 μg/ml
    Secondary Antibody:
    Streptavidin-HRP 0.2 μg/ml




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Cat. No. A02002
Size
Price
40 µg $198.00