|Description||The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRabᵀᴹ technology to generate high affinity and specific monoclonal rabbit antibodies. The HA tag is composed of several amino acids containing YPYDVPDYA and does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins.
GenScript MonoRabᵀᴹ HA tag Antibody (109B2) [Biotin], mAb, Rabbit is the biotin conjugated form of MonoRabᵀᴹ HA tag Antibody (109B2), mAb, Rabbit. This antibody is suitable for detecting the expression level of HA tag fusion proteins.
|Immunogen||A synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). conjugated to KLH|
|Purification||Protein A affinity column|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, contains 1% BSA and 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.|
|Specificity||MonoRab™ HA tag Antibody recognizes HA tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.|
|Storage||The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.|
|Note||GenScript can customize this product per customer's request including product size, buffer components, etc.|
|Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 μg/ml
Western Blot: 0.2-1 μg/ml
Other applications: user-optimized
Western blot analysis of MonoRab™ HA tag Antibody (109B2) [Biotin], mAb, Rabbit with HA-tag fusion proteins.
Lane 1 : 25 ng N-terminal HA-tagged fusion protein
Lane 2 : 10 ng N-terminal HA-tagged fusion protein
Lane 3 : 5 ng N-terminal HA-tagged fusion protein
Lane 4 : 1 ng N-terminal HA-tagged fusion protein
Lane 5: 25 ng C-terminal HA-tagged fusion protein
Lane 6: 10 ng C-terminal HA-tagged fusion protein
Lane 7: 5 ng C-terminal HA-tagged fusion protein
Lane 8: 1 ng C-terminal HA-tagged fusion protein
MonoRabᵀᴹ HA tag Antibody (109B2)[Biotin], mAb, Rabbit (A02002-40) 1 μg/ml
Streptavidin-HRP 0.2 μg/ml
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