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MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail

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MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail (A02014) is specific to Camelid IgG and have no cross-reactivity with mouse, rat, rabbit, goat and human immunoglobulins.

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail (A02014) is specific to camelid heavy chain antibodies (IgG2&3), but not to the camelid conventional antibody (IgG1).

Lane 1: VHH #1 100 ng
Lane 2: VHH #1 50 ng
Lane 3: VHH #1 25 ng
Lane 4: VHH #2 100 ng
Lane 5: VHH #2 50 ng
Lane 6: VHH #2 25 ng

Primary antibody:
MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail (Genscript, A02014, 0.5μg/ml)
Secondary antibody:
Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb (Genscript, A00098, 1:5,000)

The affinity of MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail (Cat.no A02014) with 15 random VHHs is measured by Biacore. The cocktail antibody shows comprehensive binding activity and high affinity with all of the15 VHHs.

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Overview

Synonyms	Rabbit anti-camelid VHH, single-domain antibody monoclonal antibody cocktail (Min X Ms, Rt, Rb, Gt, Hu).
Description	A camelid single-domain antibody (sdAb, called Nanobody by Ablynx, the developer) is a peptide chain of about 110 amino acids long, comprising one variable domain of a heavy-chain antibody, these are called VHH fragments. Like a whole antibody, it is able to bind selectively to a specific antigen. Single-domain antibodies allow a broad range of applications in biotechnical as well as therapeutic uses due to their small size, simple production and high affinity. GenScript MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail is a unique secondary antibody, composed of several rabbit anti-Camelid VHH monoclonal antibodies. Those antibodies were produced from hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with llama VHH antibody.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	Llama VHH antibody
Purification	Protein A affinity column

Properties

Subclass	IgG
Concentration	0.5 mg/ml, supplied in PBS (pH 7.4) with 0.02% sodium azide and 50% glycerol.
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity	GenScript MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail is specific for Camelid VHH antibody (Llama，Alpaca and Camel). It has no cross-reactivity with mouse, rat, rabbit, goat or human immunoglobulins.
Storage	Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by primary/secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western Blot: 1:1,000-1:5,000
ELISA: 1:5,000-1:10,000
Other applications: user-optimized
Optimal working dilutions must be determined by the end user.

Examples

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail (A02014) is specific to Camelid IgG and have no cross-reactivity with mouse, rat, rabbit, goat and human immunoglobulins.

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail (A02014) is specific to camelid heavy chain antibodies (IgG2&3), but not to the camelid conventional antibody (IgG1).