|Synonyms||iFluor 555 conjugated rabbit anti-camelid VHH, single-domain antibody monoclonal antibody cocktail (Min X Ms, Rt, Rb, Gt, Hu).|
|Description||A camelid single-domain antibody (sdAb, called Nanobody by Ablynx, the developer) is a peptide chain of about 110 amino acids long, comprising one variable domain of a heavy-chain antibody, these are called VHH fragments. Like a whole antibody, it is able to bind selectively to a specific antigen. Single-domain antibodies allow a broad range of applications in biotechnical as well as therapeutic uses due to their small size, simple production and high affinity.
GenScript MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail [iFluor 555] is iFluor 555 conjugated MonoRabTM Rabbit Anti-Camelid VHH Cocktail. The Cocktail is a unique secondary antibody, composed of several rabbit anti-Camelid VHH monoclonal antibodies. Those antibodies were produced from hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with llama VHH antibody.
|Immunogen||Llama VHH antibody|
|Purification||Protein A affinity column|
|Concentration||0.5 mg/ml, supplied in PBS (pH 7.4), containing 10 mg/ml BSA, 0.02% sodium azide and 50% glycerol.|
|Specificity||GenScript MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail [iFluor 555] is specific for Camelid VHH antibody (Llama, Alpaca and Camel). It has no cross-reactivity with mouse, rat, rabbit, goat or human immunoglobulins.|
|Storage||Store at -20°C and it should be protected from prolonged exposure to light. This product is stable for 1 year upon receipt, when handled and stored as instructed.|
|Note||GenScript can customize this product per customer's request including product size, buffer components, etc.|
|Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by primary/secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Other applications: user-optimized
Optimal working dilutions must be determined by the end user.
VHH-based CAR-T cells were sorted by MACS with MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail [Biotin] (Cat.no A02015) and anti-Biotin magnetic nanobeads (Miltenyi) where naïve T cells serves as negative control. Then, the CAR- T cells were stained with MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail [iFluor 555] (Cat.no A02020) and analyzed by FACS.
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