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Mouse Anti-Rabbit IgG Fc Antibody (14H9), mAb

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Overview

Synonyms	Anti-Rabbit IgG Fc Monoclonal Secondary Antibody (Min X Hu, Ms, Rt, Sh, Bv, Gt, Camel)
Description	Generally, goat anti-rabbit or donkey anti-rabbit IgG polyclonal antibodies are used as secondary antibodies to detect rabbit primary antibodies. However, since polyclonal antibodies can recognize many epitopes, the background and the cross reactivity in assays is usually high, making results hard to interpret. To minimize the background, cross reactivity, and improve the lot-to-lot consistency, a unique GenScript Mouse Anti-Rabbit IgG Fc Antibody (14H9), mAb has been developed to be used as the secondary antibody. It simply substitutes conventional reagents for unparalleled clarity in results and for high quality images in immunoprecipitation, Western Blot, ELISA, immunohistochemistry and immunofluorescence. GenScript Mouse Anti-Rabbit IgG Fc Antibody (14H9), mAb is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Rabbit IgG(H&L)
Host Species	Mouse
Conjugation	Unconjugated
Immunogen	Rabbit IgG(H&L)
Purification	Protein A

Properties

Subclass	IgG1,κ
Clone ID	14H9H10
Concentration	0.5 mg/ml, supplied in PBS (pH 7.4), containing 0.02% sodium azide and 50% glycerol.
Specificity	GenScript Mouse Anti-Rabbit IgG Fc Antibody (14H9), mAb reacts with the Fc portion of rabbit IgG but not with the Fab portion of rabbit IgG. The monoclonal antibody has no cross-reactivity with human, mouse, rat, sheep, bovine, goat or camel immunoglobulins
Storage	Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by primary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western Blotting: 1:20,000-1:40000
ELISA: 1:20,000-1:40,000
Immunohistochemistry: user-optimized
Optimal working dilutions must be determined by the end user.