|Synonyms||2019-nCoV Spike S1 Antibody, mAb, Rabbit|
|Description||SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), also known as 2019-nCoV, is a positive-sense single-stranded RNA virus. It caused coronavirus disease 2019 (COVID-19). SARS-CoV-2 contains glycosylated spike (S) protein, which is composed of S1 subunit and S2 subunit. The S1 contains a receptor-binding domain (RBD) that can bind to ACE2 receptor on target cells.|
|Immunogen||Recombinant SARS-CoV-2 Spike protein fragment|
|Clone||BS-R2B12 (monoclonal antibody)|
|Concentration||1 mg/ml, 0.2 μm filtered solution in PBS, pH 7.4.|
|Specificity||The product is specific for SARS-CoV-2 Spike Protein S1 subunit and its RBD domain.|
|Storage||Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. Avoid repeated freezing and thawing cycles.|
|Note||GenScript can customize this product per customer's request including product size, buffer components, etc.|
|Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
FACS: 10 µg/ml
Other applications: user-optimized
Standard curve of SARS-CoV-2 Spike Protein Sandwich ELISA. The SARS-CoV-2 Spike Protein Sandwich ELISA assay is developed by using MonoRabTM SARS-CoV-2 Spike S1 Antibody (BS-R2B12), mAb, Rabbit (GenScript, A02058) and MonoRabTM SARS-CoV-2 Neutralizing Antibody (BS-R2B2), mAb, Rabbit (Genscript, A02051) as the capture and detection antibodies, respectively.
In this ELISA assay, MonoRabTM SARS-CoV-2 Neutralizing Antibody (BS-R2B2), mAb, Rabbit (Genscript, A02051) was labeled with HRP.
The sensitivity of detecting SARS-CoV-2 spike protein is up to 60 pg/ml.
For more documents, please visit "Technical Support".