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Catalog Products » Other Products » Rabbit Anti-Goat IgG (H&L) [HRP],pAb

Rabbit Anti-Goat IgG (H&L) [HRP],pAb

Description HRP Conjugated Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other anibody detection methods.
Host Species Rabbit
Antigen Species Goat
Conjugation Peroxidase (Horseradish)
Immunogen Goat IgG whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum.
Storage Store Secondary Antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:50,000 - 1:100,000
Immunohistochemistry: 1:1,000 - 1:5,000
Western Blot: 1:5,000 - 1:20,000
Other Dilution: User Optimized

Rabbit Anti-Goat IgG (H&L) [HRP],pAb
Rabbit Anti-Goat IgG (H&L) [HRP],pAb

Western Blot of Rabbit anti-GOAT IgG (H&L) Antibody Peroxidase ConjugatedLane 1: Goat IgGLoad: 100 ng per laneSecondary antibody: GOAT IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RTBlock: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT




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Cat. No. A10012
Size
Price
2 mg $129.00
24 hour
 
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