Catalog Products » Other Products » Rabbit Anti-Mouse IgG (γ 1, 2a, 2b and 3 chain) [HRP],pAb

Rabbit Anti-Mouse IgG (γ 1, 2a, 2b and 3 chain) [HRP],pAb

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Overview

Description	Rabbit Anti-Mouse IgG (γ 1, 2a, 2b and 3 chain) antibody Peroxidase (Horseradish) conjugated, pAb. This Anti-Mouse IgG subclass pan reactive Secondary Antibody is is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development.
Host Species	Rabbit
Antigen Species	Mouse
Conjugation	Peroxidase (Horseradish)
Immunogen	Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins

Properties

Subclass	IgG
Concentration	Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution	Restore with deionized water (or equivalent)
Specificity	Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Applications

ELISA: 1:20,000 - 1:100,000
Immunohistochemistry: 1:1000 - 1:5000
Western Blot: 1:2000 - 1:10000
Other Dilution: User Optimized

Indirect ELISA of Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, and 3) antibody. Antigen: purified mouse IgG heavy and light chains. Coating amount: 0.1 µg per well. Primary antibody: Rabbit Anti-Mouse IgG (Gamma 1, 2a, 2b, and 3) HRP conjugated Antibody.Dilution series: 2-fold. Substrate: TMB.