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Western Blot of Rabbit anti-Mouse IgG2a antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a.Lane 3: Mouse IgG2b.Lane 4: Mouse IgG3. Lane 5: Mouse IgM.Load: 5 ng per lane.Primary antibody: Mouse IgG2a antibody at 1 µg/mL for overnight at 4°C.Secondary antibody: Rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 50 kDa and25 kDa, 50 kDa and 25 kDa for Mouse IgG2a. Other band(s): Weak reactivity for to Mouse IgG1.
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