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Rabbit Anti-Mouse IgG2a,pAb

Description Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse IgG2a heavy chain. Anti-Mouse IgG2a is ideal for investigators involved in Serum Protein Component research.
Host Species Rabbit
Antigen Species Mouse
Conjugation Unconjugated
Immunogen Mouse IgG2a heavy chain

Subclass IgG
Concentration Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Specificity Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG2a.  Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes.
Species Reactivity Mouse
Storage Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

ELISA: 1:15,000 - 1:30,000
Immunohistochemistry: 1:2,000 - 1:10,000
Western Blot: 1:4,000 - 1:20,000
Other Dilution: User Optimized

Rabbit Anti-Mouse IgG2a,pAb
Rabbit Anti-Mouse IgG2a,pAb

Western Blot of Rabbit anti-Mouse IgG2a antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a.Lane 3: Mouse IgG2b.Lane 4: Mouse IgG3. Lane 5: Mouse IgM.Load: 5 ng per lane.Primary antibody: Mouse IgG2a antibody at 1 µg/mL for overnight at 4°C.Secondary antibody: Rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 50 kDa and25 kDa, 50 kDa and 25 kDa for Mouse IgG2a. Other band(s): Weak reactivity for to Mouse IgG1.

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Cat. No. A10112
1 mg $149.00
24 hour