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Rabbit Anti-Mouse IgG (H&L) [HRP],pAb

Description Rabbit Anti-Mouse IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Mouse secondary antibody conjugated to horseradish peroxidase is available in a variety of formats. Anti-IgG Secondary Antibody conjugate is Suitable for western blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Host Species Rabbit
Antigen Species Mouse
Conjugation Peroxidase (Horseradish)
Immunogen Mouse IgG whole molecule

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Storage Store mouse second antibody conjugate at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:50,000 - 1:300,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:10,000 - 1:30,000
Other Dilution: User Optimized

  • Rabbit Anti-Mouse IgG (H&L) [HRP],pAb
  • Rabbit Anti-Mouse IgG (H&L) [HRP],pAb

    Western Blot of Rockland's Anti-Mouse IgG Antibody Peroxidase Conjugated detecting mouse IgG. Mouse IgG (1.0 mg) was separated on a 4-20% gradient gel and then transferred to Whatman Protran BA 85 nitrocellulose (0.45 µm pore size)., and blocked with 5% BLOTTO o/n at 4° C. The membrane was then probed with a 1:2,500 dilution of HRP Rb-a-Mouse IgG [H&L] (Cat. No. A10118) for 45 min at RT. FemtoMax Super Sensitive Chemiluminescent HRP Substrate. Was added and the image was captured using a BioSpectrum Imaging System with a BioChemi 500 -28C cooled 4.0 Megapixel CCD Camera from UVP (www.uvp.com, Upland, CA). Binning: 5X5. Exposure time: 2 min. Aperture open. No emission or excitation filters used.

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Cat. No. A10118
2 mg $129.00