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Western Blot of Rockland's Anti-Mouse IgG Antibody Peroxidase Conjugated detecting mouse IgG. Mouse IgG (1.0 mg) was separated on a 4-20% gradient gel and then transferred to Whatman Protran BA 85 nitrocellulose (0.45 µm pore size)., and blocked with 5% BLOTTO o/n at 4° C. The membrane was then probed with a 1:2,500 dilution of HRP Rb-a-Mouse IgG [H&L] (Cat. No. A10118) for 45 min at RT. FemtoMax Super Sensitive Chemiluminescent HRP Substrate. Was added and the image was captured using a BioSpectrum Imaging System with a BioChemi 500 -28C cooled 4.0 Megapixel CCD Camera from UVP (www.uvp.com, Upland, CA). Binning: 5X5. Exposure time: 2 min. Aperture open. No emission or excitation filters used.
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