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Western Blot of Peroxidase conjugated Sheep anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: None.Load: 100 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Other band(s): none.
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