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Catalog Products » Other Products » Sheep Anti-Mouse IgG (H&L) [HRP],pAb

Sheep Anti-Mouse IgG (H&L) [HRP],pAb

Description Anti-Mouse IgG whole molecule antibody generated in sheep detects specifically Mouse IgG whole molecule . This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Sheep
Antigen Species Mouse
Conjugation Peroxidase (Horseradish)
Immunogen Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in sheep.

Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Mouse IgG and Mouse Serum.
Species Reactivity Mouse
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:100,000 - 1:200,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:5,000
Other Dilution: User Optimized

Sheep Anti-Mouse IgG (H&L) [HRP],pAb
Sheep Anti-Mouse IgG (H&L) [HRP],pAb

Western Blot of Peroxidase conjugated Sheep anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: None.Load: 100 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase sheep secondary antibody at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Other band(s): none.




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Cat. No. A10144
Size
Price
2 mg $129.00
24 hour
 
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