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Donkey Anti-Mouse IgG (H&L) [HRP],pAb

Description Donkey Anti-Mouse IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically mouse IgG (H&L). This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Host Species Donkey
Antigen Species Mouse
Conjugation Peroxidase (Horseradish)
Immunogen Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in donkey
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Subclass IgG
Concentration Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Restore with deionized water (or equivalent)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.
Species Reactivity Mouse
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

ELISA: 1:70,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:2,000 to 1:10,000
Other Dilution: User Optimized

  • Donkey Anti-Mouse IgG (H&L) [HRP],pAb
  • Donkey Anti-Mouse IgG (H&L) [HRP],pAb

    Western Blot of Donkey anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated.Lane 1: Mouse IgG.Lane 2: None.Load: 50 ng per lane.Primary Antibody: None.Secondary antibody: Mouse IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG.Other Band(s): None.

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Cat. No. A10148
2 mg $129.00