|Description||Goat Anti-Mouse IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. F(ab')2 anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:50,000 - 1:100,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:1,000 - 1:10,000
Other Dilution: User Optimized
Western Blot of Goat anti-Fab2 Mouse IgG Antibody Peroxidase Conjugated Pre-Absorbed.Lane 1: Rabbit Serum.Lane 2: Rabbit Serum.Lane 3: Human Serum.Lane 4: Human Serum.Lane 5: Human IgG.Lane 6: Rat IgG.Lane 7: Bovine IgG.Lane 8: Horse IgG.Lane 9: Rabbit IgG.Lane 10: Mouse IgG.Load: Lane 1, 3 - 2 uL, Lane 2, 4 - 5 uL, Lanes 5 through 10 - 100 ng.Primary Antibody: noneSecondary Antibody: Goat anti-Fab2 Mouse IgG Antibody Peroxidase Conjugated Pre-Absorbed at 1:5,000 for 1 HR at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 55, 25 kDa for Mouse IgG.
For more documents, please visit "Technical Support".