|Description||Goat Anti-Mouse IgG (H&L) antibody Peroxidase (Horseradish) conjugated, pAb. Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically mouse IgG. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.|
|Immunogen||Anti-Mouse IgG (H&L) was produced by repeated immunization with mouse IgG whole molecule in goat.|
|cannot ship to canada||cannotShipToCanada|
|Concentration||Lyophilized , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Reconstitution||Restore with deionized water (or equivalent)|
|Specificity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.Ã Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.Ã No reaction was observed against anti-Papain or anti-Goat IgG F(c).|
|Storage||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|ELISA: 1:10,000 - 1:100,000
Immunohistochemistry: 1:500 - 1:2,500
Western Blot: 1:2,000 - 1:20,000
Other Dilution: User Optimized
Western blot of Peroxidase conjugated Goat Fab Anti-Mouse IgG secondary antibody.Lane 1: Mouse IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Blocking: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG.Other band(s): None.
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