The following procedure is used to minimize the pain, discomfort, or distress in animals
from the production of polyclonal antibody and monoclonal antibody.
Adjuvant
If complete Freund's adjuvant is used for the priming dose, incomplete
Freund's adjuvant
should be used for the subsequent booster injections.
Immunization
Injections is subcutaneous or, in rodents, intraperitoneal. For
multiple
sites, not
more than 0.1 ml per site is used for mouse and rats, 0.25 ml for rabbits, 0.5 ml for sheep and goats.
Sites
should be well separated to prevent consolidation of inflammatory responses.
Blood Collection
Maximum withdrawal should not exceed 5 ml/kg per week or 10 ml/kg
every other week
for all species.
Titer Sampling Routes
For rabbits, use ear vein; for mice, use tail vein; for
other
species, use
peripheral vessel.
Vasodilation
Do not use xylene or other inflammatory agents as vasodilating
agents.
Radiant heat or
warm water is the recommended alternative. Anesthesia is not required for titer sampling using
recommended
routes.
Monitoring
Investigators assume responsibility for ensuring their animals' health
through adequate
monitoring. If clinical problems arise, they must consult the veterinary staff for assistance in
managing
them, or alternatively euthanasia their animals.
Our customer service representatives are available 24 hours a day, Monday through Friday
to
assist
you.
