Overview

Recombinant protein production is not always easy and straightforward. Certain classes of proteins such as membrane proteins, toxins, and ion channel proteins, are difficult to express. As many factors can impact protein expression and purification, the optimization process is usually labor intensive and time consuming. To address some of the uncertainties and challenges, GenScript protein expression experts offer custom bacterial expression services with cutting-edge solutions, in which customers flexibly choosing an option that fits their requirements: from codon optimization to gene synthesis to small scale testing and scale up, together with protein purification and characterization. GenScript protein expression experts have successfully delivered more than 50,000 batches of recombinant proteins in E.coli system.

BacPower™ Advantages

Ideal for challenging proteins and other difficult projects
Ideal for challenging proteins and other difficult projects
Flexible and affordable service tailored to meet your demands
Flexible and affordable service tailored to meet your demands
Fast turnaround - as little as 4 weeks to get your purified proteins
Fast turnaround - as little as 4 weeks to get your purified proteins
State-of-the-art technologies
State-of-the-art technologies - GenSmart™ Codon Optimization, BacPower™ expression system, and FoldArt protein refolding technology
One-stop service platform from gene to protein
One-stop service platform from gene to protein
Available with high throughput E.coli expression service
Available with high throughput E.coli expression service.

Service Details

Milestones Specifications Deliverables Timeline*
1 Gene synthesis, Cloning, Plasmid Prep
  • Gene synthesis and Codon optimization
  • Subcloning into appropriate expression vector
  • Plasmid Prep and DNA QC
  • Optimized gene sequence report
  • Protein in buffered solution with your specified amount & purity
  • QC data
≤2 weeks
2 Protein Expression Evaluation
  • Transform plasmids into appropriate bacterial expression strain
  • Protein expression evaluation and optimization
≤1 week
3 Protein Purification, Tag Removal (Optional) & Refolding (Optional)
  • Scale up protein expression using optimized conditions
  • Purification to reach desired protein amounts and purity
  • Tag removal and separation of tag-free protein (if requested)
  • Refolding in case of insoluble protein (performed only if required)
≤1 week
4 QC & Delivery
  • Purity analysis and Detection using SDS-PAGE and Western Blot (for tagged protein)
  • Bradford assay for quantitation
  • Protein delivery
≤1 week

Note:
*All timelines are approximate. In most cases, we deliver within 4-6 weeks. However, optional steps might prolong the overall timeline slightly.
For any large scale protein production requirements, you could try our fully customizable E. coli fermentation services. Talk to our technical account managers for more details.

Case Studies

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with GenScript Custom
Protein Services

Get in Touch with GenScript Custom Protein Services
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Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.

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