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GenScript's BacuVance baculovirus / Insect cell expression system was developed by our in-house team of scientists for virus production and expression of recombinant proteins from baculovirus-infected insect cells. As the largest gene synthesis supplier in the U.S., GenScript can provide you with free, advanced codon optimization specific to Sf9, Sf21, Hi-5, and S2 insect cell lines, to further enhance the expression of your protein.
There are several advantages of using baculovirus expression system over E. coli system, such as improved solubility, ability to incorporate post-translational modifications, and higher yields for secreted proteins. For customers who prefer a eukaryotic expression system, are exploring lower-cost alternatives to mammalian expression system but do not want to compromise on the overall quality of their recombinant protein, we strongly recommend considering our BacuVance baculovirus / Insect cell expression system. Using our baculovirus expression system we can establish the best possible protocol for your custom project and then scale up to get the highest expression of your target protein.
For customers who wish to establish the best possible protocol for their project and then scale up, GenScript recommends using the sophisticated platform to explore different conditions for the expression of target proteins.
| Milestones | Specifications | Deliverables | Timeline1 | |
|---|---|---|---|---|
| 1 | Gene synthesis and subcloning |
|
Construct(s) report | 2 weeks |
| 2 | Virus generation |
|
5 ml, >107 pfu/ml virus stock | 2–3 weeks |
| 3 | Pilot expression evaluation |
|
Expression data | 1.5 weeks |
| 4 | 1 L expression and purification |
|
Purified proteins QC data |
1–2 weeks |
Note:
1. Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time.
2. Other QC data, such as LC-MS/MS coverage, N-terminal sequencing and LC-MASS are available with additional charge.
Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.
