For customers who wish to establish the best possible protocol for their project and then scale up, GenScript recommends using the sophisticated platform to explore different conditions for the expression of target proteins.
Milestones | Specifications | Deliverables | Timeline1 | |
---|---|---|---|---|
1 | Gene synthesis and subcloning |
|
Construct(s) report | 2 weeks |
2 | Virus generation |
|
5 ml, >107 pfu/ml virus stock | 2–3 weeks |
3 | Pilot expression evaluation |
|
Expression data | 1.5 weeks |
4 | 1 L expression and purification |
|
Purified proteins QC data |
1–2 weeks |
Note:
1. Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time.
2. Other QC data, such as LC-MS/MS coverage, N-terminal sequencing and LC-MASS are available with additional charge.
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