One-Stop Solutions for Antibody Drug Discovery

Therapeutic targets are identified and characterized through different approaches (e.g., bioinformatics, genetic association, expression profile, functional screening, etc.) to demonstrate how their modulation affects disease states, thus revealing their direct involvement in pathogenesis. Screening drug targets is a crucial starting point in the target discovery milestone. Furthermore, the success of target screening directly affects the downstream steps leading to drug development. Therefore, to ensure success, the following questions must be addressed: 1) What role do target molecules play in upstream and downstream pathways? 2) How are genes encoding target molecules and related proteins transcriptionally regulated? GenScript can provide customized and complete molecular solutions from gene synthesis and expression-plasmid construction to high-throughput screening library, virus packaging, and assay cell line generation to help scientists worldwide expedite the target discovery process.

GenScript Solutions:

NeoAntigen Peptides can be used for everything from the functional screening of neoantigen candidates for TCR therapy to clinical stage vaccine immunogenicity testing.

GenTitan oligo pool is widely used for gene editing libraries, mutant libraries, and NGS capture probe libraries to identify druggable targets.

CRISPR gRNA library is a genome-wide gRNA library based on CRISPR technology and oligo pool for high-throughput gene editing, enabling high-throughput target screening or functional gene screening.

Lentivirus Packaging^Upgraded is a powerful delivery vector that ensures the long-term expression of your target.

CellPower™assay cell lines are usually used for gene overexpression and inducible expression to help elucidate the functional role of drug targets.

• Case Study 1: GenTitan Mega Chip 8 Million Oligo pool construction

  Request: Oligo pool with 8,400,000 sequences, 150nt in length.

  GenScript solution: 8 million Mega chip, largest in the market, synthesized by semiconductor technology

  

  

  Results:

  8.4M custom oligo evenly distributed

  99.96%coverage rate

  Covers >99.7% oligos between log2 read count 5.5 and 10.5

  What’s more?

  GenScript provides off-the-shelf genome-scale CRISPR knock-out (GeCKO) v2 libraries and transcriptional activation SAM library to achieve high-throughput screening of functional genes. The targets, either determined through the drug-gene interaction database or pathway analysis, could be targeted and screened by pathway-focused CRISPR gRNA library.

• Case Study 2: Assay cell line for target screening

  Luciferase and RFP reporter cell line

  An in vitro model for drug screening of ER stress