Cas12a (cpf1) crRNA Synthesis Service

Reliable Service to Expand Your Gene Editing Toolbox

Overview Advantages Services Case Studies Contact Us

Home » Cas12a (cpf1) crRNA Synthesis

Overview

Cas12a (Cpf1) is a novel CRISPR-Cas DNA endonuclease that, like Cas9, can be used to create double-stranded breaks in DNA. Cas12a offers several advantages over Cas9 that expand its utility in gene editing applications:

Expanded editing scope: Cas12a recognizes T-rich PAM sequences, enabling genome editing in species with AT-rich genomes such as zebrafish, etc.
Enhanced editing efficiency: In contrast to the blunt ends created by Cas9, Cas12a produces staggered sticky ends at the 5' end, resulting in improved homology-directed repair (HDR) and gene editing efficiency.
Shorter gRNA: Cas12a crRNA is around 40-44nt, including a constant region of 20nt and a specificity-binding region of 20-24nt. Also, the crRNA does not require the use of a tracrRNA, making chemical synthesis simpler and more economical.

Leveraging over 21 years of oligonucleotide synthesis experience, GenScript offers customized Cas12a crRNA, supporting AsCas12a/LbCas12a and various customized Cas12 crRNA options, with high purity, precise molecular weight, and proven high efficiency in gene knock-out/knock-in.

30% off!
Improve editing efficiency and reduce cost with our industry-leading sgRNA

Get 30% off any order of pegRNA (SC1518-CRISPR), EasyEdit sgRNA (SC1969), SafeEdit sgRNA (SC1968), or Cas12a crRNA (SC1518-CRISPR) using the code 30%OFFGUIDE. Up to 10 nmol per guide.
Available for both desalt & HPLC purified grade. Available for online & offline ordering.
From now until Dec 31^st 2024. Terms Apply.

*Promotions exclude customers in China, Japan, France, Spain, Norway, Switzerland, Italy, Sweden, Denmark, Finland, Portugal, Iceland, Monaco and some organizations. Valid for orders placed between Sep 3^rd and Dec 31^st 2024 only and cannot be applied to existing orders.

Learn More

Advantages

Superior Quality

Precise molecular weight, high purity,
excellent batch-to-batch consistency, RUO to cGMP

Proven Efficiency

High gene knockout/knock-in efficiency
verified in multiple cell lines & loci

Flexible Solutions

Desalt and HPLC Grade
None or complimentary PS-2′-Ome modifications

Application

KO/KI Basic Research

Species rich in AT-genome, etc.

Gene & Cell Therapy

High fidelity with low off-target effects

Diagnostic Tools

DETECTR technology used for
the detection of viruses, tumor , etc.

Services

Service	Cas12a (cpf1) crRNA Synthesis Service
Length	40-49nt* *For <40nt or>50nt RNA oligo synthesis, please inquire here
Modification	Default: No modifications 2'-O-Methylation and phosphorothioate modifications optional with NO additional cost Other custom modifications available
Purification	Desalt & HPLC
Quantity	Starting from 2 nmol, up to 5000nmol, Lyophilized powder
QC Reports	COA and MS report (HPLC report available for HPLC grade)
Production Time	4 business days (Exclude shipping time)
How to Order	For AsCas12a and LbCas12a crRNA synthesis, you can directly provide a variable region sequence of 19-29 nucleotides, and we will automatically add a proven scaffold sequence to the final crRNA. For other Cas12a variants, please provide the complete 40-49 nucleotide sequence directly.

Modification Comments>>

30% off! Competitive Price!

Quantity	Desalt Grade	HPLC Grade
2 nmol	$65 $45.5	Inquiry
5 nmol	$90 $63
10 nmol	$120 $84

Need help from an expert to support your Cas12a crRNA request?

Book A Call

Email Us

Case Studies

Precise molecular weight & high purity

Dealt purified crRNA:
Precise molecular weight

HPLC purified crRNA:
Precise molecular weight

HPLC purified crRNA:
Purity up to 99.20%
High knock-out efficiency up to 98%
Results: Using GenScript's Cas12a crRNA, high gene knock-out efficiency can be achieved with different Cas12a enzymes：
- HEK293T cells: AsCas12a knock-out efficiency up to 98%, LbCas12a knock-out efficiency up to 93%, MAD7 knock-out efficiency up to 94%.
- Jurkat cells: AsCas12a knock-out efficiency up to 98%, LbCas12a knock-out efficiency up to 98%, MAD7 knock-out efficiency up to 97%.
High knock-in efficiency up to 36%
Results: Using GenScript's Cas12a crRNA, high gene knock-in efficiency can be achieved for different enzymes:
- TRAC locus: AsCas12a knock-in efficiency up to 23%, LbCas12a knock-in efficiency up to 25%, MAD7 knock-in efficiency up to 24%.
- B2M locus: AsCas12a knock-in efficiency up to 33%, LbCas12a knock-in efficiency up to 36%, MAD7 knock-in efficiency up to 30%.
Low cytotoxicity, reducing confounding factors

Results: GenScript's Cas12a crRNA does not affect the cell viability of HEK293 cells and Jurkat cells, and will not interfere with subsequent gene editing experiments.

Cas12a crRNA Design Tool

GenScript offers an easy-to-use crRNA design tool for AsCas12a and LbCas12a, which includes a variety of crRNA design parameters for your reference and selection. Try it now:

Cas12a crRNA Design Tool

Get in Touch
with GenScript CRISPR Experts

First Name

Please enter a valid first name.

Last Name

Please enter a valid last name.

Please enter a valid email name.

Industry

Please select a valid industry.

Country/Region

Please select a valid country.

Zip Code (Optional)

Company Name

Please enter a valid institution/company.

Your Message

Please enter a message in the textarea.

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission