For enriched CD4/CD8 T cell from PBMC : In this experiment, T cells were isolated from starting material of PBMCs by using a combination of CytoSinct™ CD4 Nanobeads, human (RUO) (Cat# L00863) and CytoSinct™ CD8 Nanobeads, human (RUO) (Cat# L00864), The isolated T cells were activated using GenScript’s Enceed™ T cell activation reagent (RUO) (Cat# L00899), In a parallel experiment for comparison, T cells were activated using a Competitor’s T cell activation reagent.
Figure A: After 48 hours, the expression of the activation marker (CD25 and CD69) on the cell surface was detected via flow cytometry.
Figure B: The Enceed™ T cell activation reagent activated larger percentage of the cell population compare to the reagent from competitor.
Figure C and D: T cell viability and Expansion using Enceed™ T cell activation reagent and the competitor’s product.
Figure E: Flow cytometry analysis of T cell subpopulations on day 14 between Enceed™ T cell activation reagent and competitor reagent.