GenScript Enceed™ T Cell Activation, Reagent

Key Feature

Enceed™ T cell Activation reagent induces robust and efficient activation and expansion of any start samples including isolated T cells or PBMC.

This ready-to-use reagent is gentle on T cells ensuring viability of activated cells.

The small-sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation.

Maintains Higher % of preferred phenotype.

Appropriate to use for scalable application for clinical application.

Learn more about ™ GenScript’s Enceed™ T cell Activation reagent

• CD3/CD28 T Cell Activation Expansion - For enriched CD4/CD8 T cell from PBMC

  For enriched CD4/CD8 T cell from PBMC : In this experiment, T cells were isolated from starting material of PBMCs by using a combination of CytoSinct™ CD4 Nanobeads, human (RUO) (Cat# L00863) and CytoSinct™ CD8 Nanobeads, human (RUO) (Cat# L00864), The isolated T cells were activated using GenScript’s Enceed™ T cell activation reagent (RUO) (Cat# L00899), In a parallel experiment for comparison, T cells were activated using a Competitor’s T cell activation reagent.

  

  Figure A: After 48 hours, the expression of the activation marker (CD25 and CD69) on the cell surface was detected via flow cytometry.

  Figure B: The Enceed™ T cell activation reagent activated larger percentage of the cell population compare to the reagent from competitor.

  

  Figure C and D: T cell viability and Expansion using Enceed™ T cell activation reagent and the competitor’s product.

  Figure E: Flow cytometry analysis of T cell subpopulations on day 14 between Enceed™ T cell activation reagent and competitor reagent.

• CD3/CD28 T Cell Activation Expansion - For enriched CD3 T cell from PBMC

  For enriched CD3 T cell from PBMC : In this experiment, T cells were isolated from starting material of PBMCs by using a CytoSinct™ CD3 Nanobeads, human (RUO) (Cat# L00863) and the isolated T cells were activated using GenScript’s Enceed™ T cell activation reagent (RUO) (Cat# L00899). In a parallel experiment for comparison, T cells were Isolated and activated using a Competitor’s reagent. Representative data for comparison of Activation Expansion between GenScript reagent and competitor reagent are as below.

  

  Figure A: After 48 hours, the expression of the activation marker (CD25 and CD69) on the cell surface was detected via flow cytometry.

  

  Figure C: T cell Expansion using Genscript reagent and the competitor’s product.

  Figure D: Flow cytometry analysis of T cell subpopulations on day 14 between Enceed™ T cell activation reagent and competitor reagent.