Overview

Efficient and gentle cell separation based on surface markers is essential for researching specific cell populations, and for developing life-saving cell and gene therapies.

GenScript has renowned expertise in developing magnetic bead products for bioseparation of various types of biomolecules. Our novel CytoSinct™ Cell Separation Technology uses our perfected nanoparticle empowered immunomagnetic separation technology to isolate specific cell populations of interest, combining the specificity and sensitivity of antibody-based purification with the gentleness of nanoparticles and the ease and speed of magnetic separation.

The CytoSinct™ Cell Separation Manual Kit is composed of antigen specific paramagnetic Nanobeads, Columns, and magnetic Stands. The CytoSinct™ Nanobeads are nanometer-sized, biodegradable, easy-to-use, and enable highly efficient cell isolation. CytoSinct™ Columns amplify the magnetic field and enable efficient separation with minimal labeling using the small beads. The separated cells are compatible with most downstream applications including cell culture, activation, expansion, flow cytometry analysis and translational research. Armed with the flexibility and specificity of the CytoSinct™ Cell Separation technology, you can use a variety of starting materials. Start with PBMCs or leukapheresis products. Enrich your target cells in your sample or deplete them. For every experimental application, the CytoSinct™ Cell Separation technology can provide you with the separation results you require. Even more, CytoSinct™ Cell Separation Kit is compatible with other column-based cell separation platforms. Try this great tool to advance your research by simplifying your cell separation procedure.

Schematic diagram showing the structure and size of CytoSinct™ Nanobeads.

CytoSinct™ Cell Isolation kit including Nanobeads, Columns, and Magnetic Separator.

Starting Materials

PBMCs

Downstream Applications

Cell culture, activation and expansion

Molecular investigation

Product Features

Highly Efficient

Biodegradable

Non-toxic

Compatible

Easy-to-use

Sterilized

Paramagnetic

Product Details

CytoSinct™ Cell Separation Technology Available
in GMP Grade Soon!

GenScript's Zhenjiang Biologics Manufacturing Center. Covering an area of 100,000 square meter space, the manufacturing center is designed and constructed in total compliance with the good manufacturing practice (GMP) requirements of FDA, EMEA and cFDA, and can meet the needs for Phase I to III clinical sample production and commercial production.

Efficient and gentle cell isolation based on surface markers is essential for researching specific cell populations, and for developing lifesaving cell and gene therapies. Purity, recovery and viability are among the most important characters of cells after isolation. CytoMagTM Nanobeads are nanometer-sized beads that isolate your target cells gently and efficiently, making your post-isolation cells suitable for a variety of down-stream applications. The separated cells are compatible with most downstream applications including cell culture, activation, expansion, flow cytometry analysis, and translational research.

• High Purity, Efficient Recovery, High Viability, High Specificity

Data: Leukopheresis samples were incubated with either GenScript’s CytoSinctTM CD3 Nanobeads or Competitor’s product, then isolated using column method and analyzed using flow cytometry.

Figure 1: Representative pre- and post-isolation data. Purity and recovery of CD3+ T-cells was analyzed after isolation using GenScript’s CytoMagTM CD3 Nanobeads or Competitor’s product.

Data: Human frozen PBMC samples were incubated with GenScript’s CytoSinctTM CD3 Nanobeads, isolated using column method, and analyzed using flow cytometry.

Figure 2: Representative pre- and post-isolation data using frozen PBMC as starting material. Purity, recovery, and viability of CD3+ T-cells was analyzed after isolation using GenScript’s CytoMagTM CD3 Nanobeads.

Efficient and gentle cell isolation based on surface markers is essential for researching specific cell populations, and for developing lifesaving cell and gene therapies. Purity, recovery and viability are among the most important characters of cells after isolation. CytoMagTM Nanobeads are nanometer-sized beads that isolate your target cells gently and efficiently, making your post-isolation cells suitable for a variety of down-stream applications. The separated cells are compatible with most downstream applications including cell culture, activation, expansion, flow cytometry analysis, and translational research.

• High Purity, Efficient Recovery, High Viability, High Specificity

CytoSinctTM CD4 Nanobeads shows comparable purity and recovery to Competitor’s product, as shown in Figures 1 and 2.

Data: Leukopheresis samples were incubated with either GenScript’s CytoSinctTM CD4 Nanobeads or Competitor’s product, then isolated using column method and analyzed using flow cytometry.

Figure 1: Representative pre- and post-isolation data. Purity and recovery of CD4+ T-cells was analyzed after isolation using GenScript’s CytoMagTM CD4 Nanobeads or Competitor’s product.

Data: Human frozen PBMC samples were incubated with GenScript’s CytoSinctTM CD4 Nanobeads, isolated using column method, and analyzed using flow cytometry.

Figure 2: Representative pre- and post-isolation data using frozen PBMC starting material. Purity, recovery, and viability of CD4+ T-cells was analyzed after isolation using GenScript’s CytoMagTM CD4 Nanobeads.

Efficient and gentle cell isolation based on surface markers is essential for researching specific cell populations, and for developing lifesaving cell and gene therapies. Purity, recovery and viability are among the most important characters of cells after isolation. CytoMagTM Nanobeads are nanometer-sized beads that isolate your target cells gently and efficiently, making your post-isolation cells suitable for a variety of down-stream applications. The separated cells are compatible with most downstream applications including cell culture, activation, expansion, flow cytometry analysis, and translational research.

• High Purity, Efficient Recovery, High Viability, High Specificity

Data: Leukopheresis samples were incubated with either GenScript’s CytoSinctTM CD8 Nanobeads or Competitor’s product, then isolated using column method and analyzed using flow cytometry.

Figure 1:Representative pre- and post-isolation data. Purity and recovery of CD8+ T-cells was analyzed after isolation using GenScript’s CytoMagTM CD8 Nanobeads or Competitor’s product.

Data:Human frozen PBMC samples were incubated with GenScript’s CytoSinctTM CD8 Nanobeads, isolated using column method, and analyzed using flow cytometry.

Figure 2: Representative pre- and post-isolation data using frozen PBMC as starting material. Purity, recovery, and viability of CD8+ T-cells was analyzed after isolation using GenScript’s CytoMagTM CD8 Nanobeads.

Product List

Catalog Number Product Name Package Size Capability
L00863-1 CytoSinct™ CD4 Nanobeads, human (RUO) 1 mL Up to 1X109 total MNCs
L00863-0.5 CytoSinct™ CD4 Nanobeads, human (RUO) 500 uL Up to 5X108 total MNCs
L00864-1 CytoSinct™ CD8 Nanobeads, human (RUO) 1 mL Up to 1X109 total MNCs
L00864-0.5 CytoSinct™ CD8 Nanobeads, human (RUO) 500 uL Up to 5X108 total MNCs
L00896-1 CytoSinct™ CD3 Nanobeads, human (RUO) 1 mL Up to 1X109 total MNCs
L00896-0.5 CytoSinct™ CD3 Nanobeads, human (RUO) 500 uL Up to 5X108 total MNCs
D00006 CytoSinct™ gStand 1 pc Compatible with all CytoSinct™ Magnets
D00007
Coming Soon!
CytoSinct™ gM columns 25 pc Up to 107 magnetically labeled cells from up to 2×108 total cells (each column)
D00008
Coming Soon!
CytoSinct™ gL columns 25 pc Up to 108 magnetically labeled cells from up to 2×109 total cells (each column)
D00009 CytoSinct™ M1 magnet 1 pc Holds 1 CytoSinct™ gM column
D00010 CytoSinct™ M8 magnet 1 pc Holds 8 CytoSinct™ gM columns
D00011 CytoSinct™ L1 magnet 1 pc Holds 1 CytoSinct™ gL column
D00012 CytoSinct™ L4 magnet 1 pc Holds 4 CytoSinct™ gL columns

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