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Combinatorial DNA Library Assembly Services

GenScript Builds Your Design Faster!

Combinatorial DNA libraries are powerful, high-throughput tools used in a variety of applications, including metabolic pathway and microbial strain engineering for optimizing biological systems and producing high-value chemicals, biofuels, pharmaceuticals, and sustainable food. Combinatorial DNA libraries, or combinatorial assembly libraries, are made as a set of predefined DNA parts strategically assembled in a specific arrangement.

Built on our advanced, high-throughput platforms and world-renowned expertise in gene synthesis, GenScript offers comprehensive combinatorial DNA library assembly services for various downstream screening needs to help accelerate your metabolic pathway and microbial strain engineering process.

Advantages

  •   One-stop, high-throughput solution
  •   Highly-customizable in pool or individual formats with up to 1x108 constructs containing 4 variable slots for 15 kb inserts
  •   Free storage for all your synthesized projects with CloneArk® Storage System
  •   Faster and more economical compared to your in-house operations
  •   Seamlessly assembled with advanced methods
  •   Expert advising on all project plans by our Ph.D.-level scientists

Service Features

Pooled Library Assembly Representative Library Assembly Arrayed Library Assembly
Pooled Library Assembly Representative Library Assembly Arrayed Library Assembly
Ideal For

>104 throughput screening

102 - 104 throughput screening

Testing every single design in your library

For screening platforms that are not sensitive to the presence of negative clones

Screening a pool of individual constructs with no concern for the presence of exact sequence in each clone

Example Workflow
Example Workflow Example Workflow Example Workflow
Description

Each construct contains up to 4 variable slots for inserts of up to 15 kb total length, cloned into pUC57 or custom vector with up to 4 μg in quantity.

A mixed pool of up to 108 different constructs, delivered in 1.5 ml tubes

Up to 10,000 individual, randomly-picked constructs out of a pool of assembled constructs, delivered in
1.5 ml tubes or 96-well plates

Up to 10,000 individual, precisely-assembled constructs based on the exact design, delivered in 1.5 ml tubes or 96-well plates

A glycerol stock of all library transformants is available upon
request

A glycerol stock of all library transformants is available upon
request

Quality Control

PCR verification of more than 48
clones to determine positive rate

PCR verification of all delivered constructs containing all designed
parts

Sequence verification of all delivered constructs

Sequence verification of 24 positive clones with a guarantee on more than 85% diversity

Sequence verification of 24 positive clones with a guarantee on more than 85% diversity

How to Order

Download and complete the excel form below and email it back to gene@genscript.com.

Pooled Library Assembly
Quote/Order Request Form

Representative Library Assembly Quote/Order Request Form Arrayed Library Assembly
Quote/Order Request Form

You can use our custom-built combinatorial DNA libraries in a variety of applications across many disciplines of life sciences, from plant biology to drug development. The following lists three major areas of combinatorial DNA library applications:

Synthetic Biology

  • Metabolic Pathway and Microbial Strain Engineering: screen and optimize your synthetic molecular pathways to develop the most efficient metabolic gene circuits for chemical, drug, and biofuel production.
  • Genome Engineering: screen high-throughput libraries of synthetic nuclease- or CRISPR/Cas9-based constructs for highly effective genome editing.

Protein Engineering

  • Create new multi-domain proteins for structural or functional screening.
  • Optimize protein function through directed evolution.
  • Generate recombinant protein or chimeric antibody libraries for high-throughput screening for biologic drug discovery and development.

Gene Expression Regulation

  • Determine the optimal combination of promoter, ribosome binding site, terminator, and other regulatory elements for your construct or expression system.
  • Create a library of reporter-gene constructs for systematic promoter analysis.
  • Express any gene of interest under the control of different promoters, e.g. for inducible or tissue-specific manipulation of lethal or developmentally vital genes.

Technical Support, Quote & Ordering Information

To discuss your project with our Ph.D. scientists and choose the best service for expediting the build phase of your project, contact us 24/7 via:

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