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Home » Gene Synthesis & Molecular Biology Services » Combinatorial DNA Libraries
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Combinatorial DNA Library Assembly Services

GenScript Builds Your Design Faster!

Combinatorial DNA Library Assembly Services

Combinatorial DNA libraries are powerful, high-throughput tools used in a variety of applications, including metabolic pathway and microbial strain engineering for optimizing biological systems and producing high-value chemicals, biofuels, pharmaceuticals, and sustainable food. Combinatorial DNA libraries, or combinatorial assembly libraries, are made as a set of predefined DNA parts strategically assembled in a specific arrangement.

Built on our advanced, high-throughput platforms and world-renowned expertise in gene synthesis, GenScript offers comprehensive combinatorial DNA library assembly services for various downstream screening needs to help accelerate your metabolic pathway and microbial strain engineering process.

Advantages

  •   One-stop, high-throughput solution
  •   Highly-customizable in pool or individual formats with up to 1x108 constructs containing 4 variable slots for 15 kb inserts
  •   Free storage for all your synthesized projects with CloneArk™ Storage System
  •   Faster and more economical compared to your in-house operations
  •   Seamlessly assembled with advanced methods
  •   Expert advising on all project plans by our Ph.D.-level scientists

Service Features

We offer three different library types to suit your individual project needs. Each assembled construct contains up to 4 variable slots for inserts of up to 15 kb total length, cloned into your vector of choice, with up to 4 ug in yield.

Pooled Library
Representative Library
Arrayed Library
Library Description Mixed pool of up to 108 different constructs Up to 10,000 individual, randomly-picked constructs out of a pool of assembled constructs Up to 10,000 individual, precisely assembled constructs based on the exact design
Ideal For Screening platforms not sensitive to presence of negative clones Screening a pool of individual constructs with no concern for presence of exact sequence in each clone Screening every single design in the library
Screening >104 throughput screening 102-104 throughput screening Every single design in the library
Deliverables 1.5 ml tubes 1.5 ml tubes or 96-well plates 1.5 ml tubes or 96-well plates
Quality Control
  • PCR verification of >48 clones to determine positive rate
  • Sequence verification of 24 positive clones
  • PCR verification of all delivered constructs containing all designed parts
  • Sequence verification of 24 positive clones
Sequence verification of all delivered constructs

Example Workflow

Pooled Library Assembly Representative Library Assembly Arrayed Library Assembly
Example Workflow Example Workflow Example Workflow

Over the last several years, GenScript has partnered with researchers in both industry and academia to build sophisticated combinatorial DNA libraries. These highly-customized libraries were used to develop new drugs, enzymes, or to discover novel gene functions and networks. The following is one example of such successful partnerships:

How to Order

Download and complete the appropriate request form below and email it back to gene@genscript.com. Our Ph.D. scientists will be in touch within 24 hours to discuss your project.

Pooled Library Assembly
Quote/Order Request Form

Representative Library Assembly Quote/Order Request Form Arrayed Library Assembly
Quote/Order Request Form

For questions or help with choosing the best library service to expedite the build phase of your project, contact us at: