The impact that the COVID-19 pandemic has had worldwide is of unprecedented proportions, with over 4.6 million infected and ~300,000+ deaths in 216 countries and areas*. As this pandemic continues to devastate global communities, the need to rapidly develop effective solutions to prevent and treat this disease is of critical importance.
From diagnostics to drug discovery and vaccine development, GenScript has developed a comprehensive range of products and services that scientists can use to accelerate COVID-19/SARS-CoV-2 research and development.
*Data current as of 5/18/2020
Services and Products
SARS-CoV-2 qRT-PCR Detection Assay Hot!
- The qRT PCR detection assay is based on GenBank Sequence NC_045512.2. This assay has successfully detected SARS-CoV-2 in positive control samples ORF1ab, RdRP, N and E genes.
- Customized detection primers and probes for all 4 genes.
Serology SARS-Cov-2 detection kits
Protein Reagents and Products
Plasmids for SARS-CoV-2 Detection and Research In-stock
- Positive control plasmids of qPCR detection assay
- Genes encoding the surface glycoprotein nucleocapsid phosphoprotein
- Homo sapiens angiotensin I converting enzyme 2 (ACE2) gene and transmembrane protease, serine 2 (TMPRSS2) for vaccine and antibody development
Stable Cell Lines
Pseudovirus Particles
- Pseudovirus particles serve as a safer alternative to conventional approaches. We offer different pseudovirus packaging options from a wide rang of SARS-CoV-2 vectors and bioluminescent tags.
Peptides and Peptide Library
- A series of SARS-CoV-2 Peptide Libraries based on GenScript's rich experience in peptide design and synthesis. These SARS-CoV-2 Peptide Libraries can be used for T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion and Cellular immune response.
How to detect the SARS-CoV-2 virus?
Principle of novel coronavirus detection
The virus detection method can be basically illustrated as below.
Step 1:
Isolate the novel coronavirus (2019-nCoV) from the patients and sequence its genome.
Step 2:
Compare the genome sequence of 2019-nCoV with human genome to find out the specific sequence in the virus genome.
Step 3:
Design PCR amplification primers and fluorescent probe primers for detecting the specific sequences identified in step 2.
Step 4:
Extract RNA from suspected individual’s serum, and convert the RNA into cDNA. The cDNA is then used as template and mixed with the PCR primers and probes for amplification. If the fluorescence signal increases rapidly and Ct value is less than 37, it can be determined as positive; If there’s no fluorescence detected, or the fluorescence signal grows slowly and Ct value finally ends up above 40, it can be determined as negative.
Figure 1: Illustration of virus entering the body and interacting with the host cell.
Figure 2: (a) The topology of the S protein (b) SARS-CoV-2 RBD sequence and structure (highlighted in red is the RBM sequence). Figure reproduced from Yu, J. et al.1
Reference:
Lan, J., Ge, J., Yu, J. et al. Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor. Nature 581, 215–220 (2020). https://doi.org/10.1038/s41586-020-2180-5
