Overview

Synthetic single guide RNA (sgRNA) is sgRNA that is chemically synthesized using the latest technological advancements. Utilizing synthetic sgRNA is a simple and cost-effective way to improve your CRISPR editing, as it offers higher editing efficiency, lower cytotoxicity, and higher lot-to-lot stability compared to IVT sgRNA.

GenCRISPR synthetic sgRNAs are proven effective for targeted gene editing in knockout and HDR mediated knock-in experiments. Combining sgRNA with Cas9 protein into a ribonucleoprotein (RNP) complex offers additional advantages over traditional plasmid or virus based delivery systems, including lower immunogenicity and reduced off-target effects.

Additionally, we offer custom synthesis of pegRNA for prime editing and crRNA for Cpf1/Cas12a editing systems.

New lower pricing!
Quantity EasyEdit sgRNA SafeEdit sgRNA cCMP/GMP-like sgRNA
2 nmol $79 $119 Learn More
4 nmol $149 $209
10 nmol $299 $459
50 nmol $839 $1299
100 nmol $1299 $1899

Ready to get started? Access our intelligent gRNA Design Tools below to design and order guide RNA and DNA templates for your CRISPR/Cas9 knockout or HDR knock-in experiments. Simply select your experiment type below to start designing:

Already have your sgRNA design? Use our simple sgRNA order form to order or get a quote:

Enter your sequence

Need help from an expert?

For a limited time, take advantage of two new promotions on our industry-leading sgRNA!

  • Get one free sgRNA! New sgRNA customers can request one free EasyEdit™ sgRNA (SC1969, 1 item, 2nmol) by using the code 'FREEGUIDE.’ Shipping fees not included.
  • Double your discovery with our CRISPR BOGO promotion! For any EasyEdit™ sgRNA or SafeEdit™ sgRNA order (SC1968 or SC1969), get another sgRNA for free (of the same or lesser grade/quantity) by using the code 'EEGUIDEBOGO’ or ‘SEGUIDEBOGO.’

From now until August 31st. Terms apply.

What synthesis options are available?

EasyEdit

Synthetic sgRNAs with modifications for reliable gene editing
1
SafeEdit

HPLC purified for improved performance
2
cGMP/GMP-like

 Supporting IND filing and clinical trials
3

EasyEdit

icon-trumpetSynthetic EasyEdit sgRNAs now starting at only $79/2nmol!

✔ Option to deliver in 96-well plates supporting high throughput screening

Most economical solution icon

Most economical solution

Ideal for early research & discovery
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High editing efficiency

Regardless of cell type
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Flexible at scale

1 to 1000+ sgRNAs 2 to 100+ nmol
  • Four reasons to choose EasyEdit sgRNA:

    Why choose EasyEdit sgRNA?

    Reliable and Consistent Editing Results

    GenScript EasyEdit sgRNA Editing Results

    GenScript EasyEdit sgRNA achieved >80% KO efficiency for all three gene targets tested, comparable to other vendors. sgRNAs were designed for 3 randomly selected gene targets (Seq_1, Seq_2, Seq_3), and KO efficiency of sgRNAs from different vendors were examined in Jurkat cells.

    Superior purity compared to competitor products

    GenScript EasyEdit sgRNA Superior purity

    GenScript EasyEdit sgRNA demonstrates higher purity compared to competitor products. UPLC analysis of sgRNA purity from different vendors for sgRNAs designed for 3 randomly selected gene targets (Seq_1, Seq_2, Seq_3).

    Higher % of full-length sgRNA sequences
    compared to competitor products

    Higher % of full-length sgRNA sequences- MS

    Higher % of full-length sgRNA sequences- NGS

    GenScript EasyEdit sgRNA exhibited minimal non-specific peaks in MS analysis (a) and a higher percentage of full-length sgRNA sequences in NGS analysis (b) compared to competitor products for the same sgRNA sequence design.

    Pricing

    Product Quantity Pricing Quote
    EasyEdit sgRNA 2 nmol $79 Quote
    4 nmol $149
    10 nmol $299
    50 nmol $839
    100 nmol $1,299

    Add-On Items

    Product Quantity Pricing Quote
    Custom Primer for Assessing Editing Efficiency 2 nmol $1.2/nt Quote
    EasyEdit Human HPRT Positive Control sgRNA 2 nmol $39 Quote
    SafeEdit Human HPRT Positive Control sgRNA 2 nmol $69 Quote
    Human HPRT Primer Mix for Assessing Editing Efficiency 2 nmol $10 Quote
    GenCRISPR™ Ultra NLS-Cas9 Nuclease (10 mg/ml) 500 μg $695 Quote
    1 mg $1181.50
    GenCRISPR™ Ultra eSpCas9-2NLS Nuclease (10 mg/ml) 500 μg $1245 Quote
    1 mg $2116.50
    Long Single-Stranded DNA Synthesis for Gene Knock-In (151-5000 nt) 3 μg and up Starting at $0.8/nt Quote

Let us suggest sgRNA designs for you:

Already have your sgRNA design?

EasyEdit
The easy yet effective one-step solution
Starting at $79/2nmol!

HPLC purified sgRNAs minimize off-targeting and cytotoxicity

✔ More than 90% purity guaranteed

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Minimal impact for
cell viability

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Reduced off-targeting from
truncated guides

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Ideal for primary cells
& stem cells

  • Two reasons to choose SafeEdit sgRNA:

    Two reasons to choose SafeEdit sgRNA:

    HPLC purified sgRNA eliminates off-targeting from truncated oligos

    SafeEdit sgRNA ensures purity

    High purity SafeEdit sgRNA ensures minimal impact for cell viability

     SafeEdit sgRNA ensures cell viability

    GenScript provides add-on QC options to ensure sustained quality of your cells:

    • Mycoplasma testing (qPCR)
    • Endotoxin testing (Kinetic LAL)
    • Stability testing
    • Cytotoxicity testing (co-culture with client specified cell line)

    Pricing

    Product Quantity Pricing Quote
    SafeEdit sgRNA 2 nmol $119 Quote
    4 nmol $209
    10 nmol $459
    50 nmol $1,299
    100 nmol $1,899

    Add-On Items

    Product Quantity Pricing Quote
    Custom Primer for Assessing Editing Efficiency 2 nmol $1.2/nt Quote
    EasyEdit Human HPRT Positive Control sgRNA 2 nmol $39 Quote
    SafeEdit Human HPRT Positive Control sgRNA 2 nmol $69 Quote
    Human HPRT Primer Mix for Assessing Editing Efficiency 2 nmol $10 Quote
    GenCRISPR™ Ultra NLS-Cas9 Nuclease (10 mg/ml) 500 μg $695 Quote
    1 mg $1181.50
    GenCRISPR™ Ultra eSpCas9-2NLS Nuclease (10 mg/ml) 500 μg $1245 Quote
    1 mg $2116.50
    Long Single-Stranded DNA Synthesis for Gene Knock-In (151-5000 nt) 3 μg and up Starting at $0.8/nt Quote

Let us suggest sgRNA designs for you:

Already have your sgRNA design?

cGMP/GMP-like
Supporting IND filing and clinical trials

GenScript now offers full cGMP and GMP-like sgRNA

✔ Supporting IND filing and clinical trials

production facility icon

State-of-the-art production facility

Clean suite with class A isolator in a class C background

QAQC icon

Comprehensive QA/QC

Proprietary NGS method to verify sgRNA identity

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Trusted by scientific partners globally

Successfully delivered 30+ cGMP grade batches

Prime editing guide RNA (pegRNA)

What is pegRNA?

Prime editing guide RNA (pegRNA), one of two components required for prime editing, is a guide RNA capable of both identifying a target site to be edited and encoding a new genetic sequence to replace a targeted sequence.

Prime editing (PE), developed by the Liu group at the Broad Institute in 2019, is a novel gene editing technique that allows for precise and efficient DNA editing by introducing a single-stranded DNA break ( or ‘nick’) in the target DNA. The system combines an engineered prime editor protein, containing a modified Cas9 nuclease fused to a reverse transcriptase enzyme, with a pegRNA, composed of a spacer sequence, sgRNA scaffold, and a reverser transcription template containing the edited bases and primer-binding site required for the desired point mutation, insertion, or deletion. This system allows the enzyme to not only cut the DNA at a specific location, but also to copy a new DNA sequence into the cut site.

Given its lack of reliance on the double-stranded DNA breaks utilized in other editing systems such as CRISPR-Cas9, prime editing has the potential to offer significantly lower off-target activity and higher editing efficiency compared to homology-directed repair.

GenScript supplies long custom guide RNAs for applications such as prime editing (pegRNA). Our expert RNA synthesis group regularly delivers projects up to 180nt at scale and purity levels to meet your unique needs.

Simply enter your full pegRNA sequence to receive a quote or contact us for design help.

Cas12a/Cpf1 guide RNA (crRNA)

What is Cas12a crRNA?

Cas12a cRNA is a specifically-designed guide RNA that is used by CRISPR-associated protein 12a (Cas12a, also known as Cpf1) to target DNA in a specific and programmable manner. Cas12a nuclease and crRNA target and cut DNA sequences in a staggered break with a 4 or 5-nt 5’ overhang, producing a cohesive end (or ‘sticky end’).

Compared to the blunt ends produced by Cas9 nuclease, these cohesive ends can make it easier to insert new genetic material into the cut site and are easily joined back together by a DNA ligase. The CRISPR-Cas12a system also benefits from not requiring a tracrRNA, as the CRISPR-Cas9 system does. For these reasons and more, CRISPR-Cas12a has potential for use in a variety of applications such as the development of gene therapies.

GenScript proudly offers custom synthesis of Cas12a crRNA. Simply click the button below to receive a quote or contact us for design help.

Testimonials

Dr. Xinlai ChengDr. Xinlai Cheng

Buchmann Institute for Molecular
Life Sciences,
Goethe University Frankfurt

"The [sgRNA] offered here truly shine and are worth every penny.... their performance and quality are unmatched, making them a valuable investment"

Resources

CRISPR/Cas9 gRNA Vector
RNP user manual

A guide on how to use CRISPR RNP for targeted genome editing.

Free Download
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CRISPR Knock-in Comprehensive Guide

Step-by-step instructions for your CRISPR knock-in experiments.

Free Download
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GenCRISPR gRNA & HDR Template Design Tools

For knockout and knock-in designs, with off-target analysis.

Design Now

FAQs

  • Technical questions

  • Design tool questions

  • Experiment questions

  • How many sgRNA or crRNA sequences are needed for targeted knock-out?

    A minimum of three (3) crRNA sequences are recommended to ensure knock-out and experimental accuracy. Independently obtained knock-out mutants provide redundancy to safeguard against any hidden off-target effects.

  • What are the advantages of using synthetic sgRNA for gene editing compared to the traditional plasmid- or virus-based gRNA delivery methods?

    Unlike the traditional plasmid or lentiviral delivery methods, CRISPR/Cas9 ribonucleoprotein (RNP) are delivered as intact complexes and do not require cellular expression. This method thus has many advantages:

    • Faster manufacturing: Synthetic sgRNA can be made in as little as three (3) business days using chemical synthesis and arrives ready to use- compared to a week or more of lab work required to produce gRNA via plasmid or lentiviral delivery.
    • DNA-free: Avoiding the delivery of foreign DNA into the cell eliminates any opportunity for transgene integration into the cell genome.
    • Detectable at high levels shortly after transfection: Editing activities can take place more quickly, as synthetic sgRNA doesn't need to be transcribed in the cell.
    • Quickly cleared from the cell for less off-target effects: The Cas-sgRNA RNP delivery vehicle is expressed transiently and degrades quickly, limiting the potential for any off-target editing.
    • Highly efficient even in hard-to-transfect cells: Experimental results have demonstrated high editing efficiency using synthetic sgRNA, regardless of the cell type used.
    • High packaging capacity: Our synthetic sgRNA can accommodate a length of up to 200nt.
    • Low immunogenicity (ideal for in vivo studies): Synthetic sgRNA consistently demonstrates lower immunogenicity and toxicity in edited cells compared to gRNA produced via plasmid or lentiviral delivery.
  • What is the difference between using synthetic sgRNA versus using a crRNA:tracrRNA duplex?

    When using sgRNA, there is no need for a crRNA:tracrRNA annealing step prior to use. More importantly, several studies have showed that sgRNA has better stability than crRNA:tracrRNA when duplexed with Cas9, thus leading to higher editing efficiency.1,2

    1. Hendel, et al., Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nat. Biotechnol., 33 (2015) 985-989

    2. Ryan et al., Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs. Nucleic Acids Research, 46 (2018) 2: 792–803

  • What is the advantage of synthetic sgRNA compared to in vitro-transcribed (IVT) sgRNA?

    Synthesis of 100 nt long sgRNAs was traditionally possible through in vitro transcription (IVT) using phage RNA polymerase. These in vitro-transcribed sgRNAs contain a 5’-triphosphate, which was thought to trigger immune response in many cell types. A recent study showed that sgRNAs with 5’-triphosphate modifications produced through in vitro transcription can indeed induce innate immune responses and lead to cytotoxicity in human and murine cells. However, chemically synthesized sgRNAs without the 5’-triphosphate modifications (such as GenCRISPR Synthetic sgRNAs) demonstrated much better editing efficiency in cells, thus supporting that chemically synthesized sgRNAs are the most ideal reagent for CRISPR genome editing currently available.3

    3. Kim et al. CRISPR RNAs trigger innate immune responses in human cells. Genome Res. 2018. 28: 367-373.

  • Why should I choose modified sgRNA versus unmodified versions?

    Our modified sgRNA has 2’-O-methyl and phosphorothioate modifications at the first three 5’ and 3’ terminal RNA residues. 2′ O-Methyl oligo modification is best characterized as an RNA analog which offers stability against hydrolysis and nucleases. The phosphorothioate (PS) modification renders the internucleotide linkage more resistant to nuclease degradation.

  • What container and delivery formats are available?

    Our sgRNA can be delivered in either single tubes or 96-well plates, formatted as dry powder or suspended in TE buffer (pH 8.0, 100 µM) or nuclease-free water (100 µM).

  • What is the maximum sgRNA length that you can chemically synthesize?

    We can chemically synthesize sgRNA up to 200 nt in length.

  • What is the maximum quantity of sgRNA that can be ordered? Do you offer arrayed sgRNA libraries?

    We can deliver chemically synthesized sgRNAs in an arrayed format, which is delivered in a 96-well plate. Each well contains custom-designed sgRNA targeting one gene of interest.

  • What grades of sgRNA do you have? Do you have GMP? Which type should I choose?

    We offer sgRNA in grades ranging from RUO to cGMP:

    EasyEdit sgRNA is purified by an optimized desalt method. Fast delivery and competitive pricing makes it ideal for screening or early stages of research.

    SafeEdit sgRNA is purified by HPLC, resulting in a guaranteed minimum of 90% purity. This option is ideal for the validation stage of development.

    For more information on our cGMP sgRNA, visit this page.

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