GenCRISPR Synthetic sgRNA

Prime editing guide RNA (pegRNA)

What is pegRNA?

Prime editing guide RNA (pegRNA), one of two components required for prime editing, is a guide RNA capable of both identifying a target site to be edited and encoding a new genetic sequence to replace a targeted sequence.

Prime editing (PE), developed by the Liu group at the Broad Institute in 2019, is a novel gene editing technique that allows for precise and efficient DNA editing by introducing a single-stranded DNA break ( or ‘nick’) in the target DNA. The system combines an engineered prime editor protein, containing a modified Cas9 nuclease fused to a reverse transcriptase enzyme, with a pegRNA, composed of a spacer sequence, sgRNA scaffold, and a reverser transcription template containing the edited bases and primer-binding site required for the desired point mutation, insertion, or deletion. This system allows the enzyme to not only cut the DNA at a specific location, but also to copy a new DNA sequence into the cut site.

Given its lack of reliance on the double-stranded DNA breaks utilized in other editing systems such as CRISPR-Cas9, prime editing has the potential to offer significantly lower off-target activity and higher editing efficiency compared to homology-directed repair.

GenScript supplies long custom guide RNAs for applications such as prime editing (pegRNA). Our expert RNA synthesis group regularly delivers projects up to 180nt at scale and purity levels to meet your unique needs.

Simply enter your full pegRNA sequence to receive a quote or contact us for design help.

Cas12a/Cpf1 guide RNA (crRNA)

What is Cas12a crRNA?

Cas12a cRNA is a specifically-designed guide RNA that is used by CRISPR-associated protein 12a (Cas12a, also known as Cpf1) to target DNA in a specific and programmable manner. Cas12a nuclease and crRNA target and cut DNA sequences in a staggered break with a 4 or 5-nt 5’ overhang, producing a cohesive end (or ‘sticky end’).

Compared to the blunt ends produced by Cas9 nuclease, these cohesive ends can make it easier to insert new genetic material into the cut site and are easily joined back together by a DNA ligase. The CRISPR-Cas12a system also benefits from not requiring a tracrRNA, as the CRISPR-Cas9 system does. For these reasons and more, CRISPR-Cas12a has potential for use in a variety of applications such as the development of gene therapies.

GenScript proudly offers custom synthesis of Cas12a crRNA. Simply click the button below to receive a quote or contact us for design help.

Resources

RNP user manual

A guide on how to use CRISPR RNP for targeted genome editing.

Free Download

CRISPR Knock-in Comprehensive Guide

Step-by-step instructions for your CRISPR knock-in experiments.

Free Download

Application of NGS-Based Technology in sgRNA Sequencing QC

Free Download

GenCRISPR gRNA & HDR Template Design Tools

For knockout and knock-in designs, with off-target analysis.

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