GenScript's plasmid DNA preparation service, customized to your desired quality and quantity needs, offers the most stringent quality control standards on the market. Preps are offered at ≥ 90% supercoil and ≤ 0.01 EU/µg endotoxin levels.
| |
Specification |
Analytical Method |
| Appearance |
Clear, no visible particles |
Visual inspection |
| A260/280 |
1.8-2.0 |
Nanodrop UV absorption |
| Concentration |
On request, default 1 mg/ml |
UV absorption |
| Supercoil content |
For information only |
Densitometry upon agarose gel electrophoresis |
| Residual RNA |
Not visible |
Visual inspection upon electrophoresis |
| Genomic DNA |
Not visible |
Visual inspection upon electrophoresis |
| Restriction analysis |
Conforming to reference pattern |
Enzyme digestion and electrophoresis |
| Endotoxin level |
Based on grade |
Limulus Amebocyte Lysate (LAL) assay |
| Quantitative LAL assay, Optional New!!! |
| Bioburden |
No growth on agar plate after 48 hours |
Bioburden assay |
For more information about our QC measures, click here.
It is time to choose the optimal grade for your DNA plasmid based on downstream application!
| Research-Grade |
- Molecular cloning
- Mutagenesis
- Southern blotting
- Library construction
- Transformation (in E.coli, bacillus, mold, and yeast)
- Protein manufacturing (in E. coli and yeast)
|
| Industrial-Grade |
- Transfection and co-transfection (in mammalian cells)
- Virus packaging
- Protein manufacturing (in mammalian cells)
- Antibody production
- Gene, vaccine, and gene therapy studies
- Animal studies
|
Frequently Asked Questions
Q: What is "animal-free cultivation media"? What is the advanced animal-free prep process and when would I need it?
A: For our research and industrial grade plasmid prep services, we use a cultivation media without any animal-derived components, making the media "animal-free." Unlike the traditional plasmid prep that uses bovine pancreatic RNase, our animal-free prep process uses a recombinant RNase that is guaranteed bovine-free. The advanced animal-free prep process is critical for clinical applications and animal studies to avoid safety risks.
Q: Why is it important to measure the supercoiled content of a DNA plasmid prep?
A: The supercoiled state of a DNA plasmid corresponds to its functional or active state in an application: the higher the supercoil level, the closer the structure of the prepared DNA plasmid to natural DNA, and hence, its better functionality. Multiple studies have shown that highly supercoiled plasmids are key to the success of numerous in vivo and in vitro applications. For example, supercoiling can strongly influence transfection efficiency or can lead to sides effects in DNA therapy. In general, the supercoil percentage has been determined as a predictor of plasmid performance and is recognized by many regulatory entities.
Q: How do endotoxins affect my research goals?
A: Endotoxins can have many adverse downstream effects on your research projects, ranging from decreased transformation efficiencies to adverse side-effects in animal studies. To learn more about endotoxins and other QC details click here.
Customer Testimonial
"We used GenScript's plasmid preparation service to prepare large quantities of plasmids that contain our Southern blot probe fragments. This saved our lab considerable time and expense. From the initial order to service delivery, GenScript provided our lab with timely follow-ups and valuable advice. We now have an adequate supply of these probes with no need to grow bacterial cultures every time a new probe is required. Thank you, GenScript!"
― Dr. Melissa A. Melan, University of Pittsburgh Medical Center, USA
Selected Publications
- Glenn K, Smith KS. Allosteric Regulation of Lactobacillus plantarum Xylulose 5-Phosphate/Fructose 6-Phosphate Phosphoketolase (Xfp). J Bacteriol. 2015 Apr;197(7);1157-63
- Petrillo C, Cesana D, Piras F, et al. Cyclosporin a and rapamycin relieve distinct lentiviral restriction blocks in hematopoietic stem and progenitor cells. Mol Ther. 2015 Feb;23(2);352-62
- Simons C, Rash LD, Crawford J, et al. Mutations in the voltage-gated potassium channel gene KCNH1 cause Temple-Baraitser syndrome and epilepsy. Nat Genet. 2014 Dec;47(1);73-7
See more recent peer-reviewed publications that cite GenScript's Plasmid Prep. Services. »