Overview

GenScript's Plasmid DNA Preparation Service is accommodating to both small research labs as well as large-scale manufacturing biotech and pharmaceutical companies, providing you with high quality plasmids with 100% full insert sequence accuracy. Choose from a variety of our packages to help you achieve highly efficient cell transfection, and to support your DNA vaccine development, antibody production, and other preclinical research projects. Request a quote now for New plasmids to be synthesized, or Re-prep of previously synthesized plasmid.

Grades and Applications

With close to 20 years of CRO experience, GenScript truly understands your needs. From transformation to extraction, we have developed a robust protocol with years of process optimization to provide you top-notch quality plasmid preps with fully customizable services.

plasmid icon

Research Grade

Research grade plasmid DNA supports preps for cloning, mutagenesis and transformation. From microgram- to gram-scale with reliable quality and fast turnaround time to accommodate tight deadlines.
plasmid icon

Industrial Grade

Industrial Grade Plasmid DNA to deliver highly efficient cell transfection with highly homogenous (≥90±10% supercoiled) plasmids. 100% sequence accuracy guaranteed!

plasmid icon

Pre-clinical Grade

Preclinical grade plasmid DNA with the most comprehensive and stringent quality specifications. Highly scalable from 10mg and up. Enjoy customizable options like animal-free production, AAV-ITR service and add-on QC options.

Research Grade Industrial Grade HT Grade Pre-clinical Grade
Price From $39 From $99 From $29 From $1199
Volume 100 µg-2 g 100 µg-2 g 10 µg-30 µg 10 mg-2 g
Turnaround Time From 2BD (bundled) From 8BD
(stand alone)
From 2BD (bundled) From 8BD
(stand alone)
From 2BD (bundled) From 9BD
(stand alone)
From 2BD (bundled) From 9BD
(stand alone)
Homogeneity Predominantly supercoiled ≥90±10% supercoiled ≥90±10% supercoiled ≥90% supercoiled
Endotoxin - Quantitative LAL assay
<0.01 EU/µg
Quantitative LAL assay
<0.1 EU/µg
Quantitative LAL assay
≤0.01 EU/µg
or
≤0.005 EU/µg
Residual RNA Non-detectable by gel electrophoresis at 200ng
Residual E. coli DNA Non-detectable by gel electrophoresis Quantitative PCR
≤ 5%
Residual Host Protein - HCP ELISA ≤1%
Bioburden Testing - No growth on agar plate after 48 hours
Animal Free Production* Available with additional charge
Enzyme Free Production - Available with additional charge
Advanced Endotoxin Removal <0.005 EU/µg - Available with additional charge
Mycoplasma Contamination - Available with additional charge
Material Archiving - Available with additional charge
Appearance Clear, colorless, no visible particulates
A260:280 1.8 - 2.0
pH - 8.0 ± 0.5 (in TE buffer)
7.4 ± 0.5 (in PBS buffer)
(5-7) ± 0.5 (in ddH2O buffer)
Restriction Analysis Conforming to reference pattern

Note: *:For research, industrial and HT grade plasmid preps: Since densitometry is semi quantitative, the genomic DNA is considered nonvisible if the comparative intensity of genomic DNA < 15% of plasmid band. Quantitative genomic DNA measurement by qPCR is available with additional charges.
**: Sequence verification of the full gene insert only applies when bundled with gene synthesis (an insert to be cloned into a plasmid).
***: Percentage of supercoiled plasmid may vary when analyzing with different methods or can be altered due to shipping conditions.
Additional QC available upon request

Additional Resources

industrial grade plasmid flyer
ITR Sequencing service

Learn more about GenScript’s proprietary ITR sequencing platform

Free Download Free Download
industrial grade plasmid flyer
Gene Therapy Tidbits

Learn how improved ITR integrity can impact AAV plasmid expression and accelerate your gene therapy pipeline!

Free Download
industrial grade plasmid flyer

A 100% Full Insert Sequence Accuracy, Guaranteed

preclinical grade plasmids flyer

With the most comprehensive and stringent QC
Tailored for preclinical studies

GLP Plasmid Prep Services

As a leading biotech company and the largest gene synthesis supplier in the USA, GenScript has accumulated a wealth of experience in GLP-level plasmid preparation.
We are happy to offer GLP plasmid prep services to provide you with ultra transparent documentation system and communication.

plasmid glp icon

Strict

Operating Procedurces

plasmid glp icon

Comprehensive

Final Report

plasmid glp icon

Data Archive

Management

Testimonials

KansasState Dr. Melissa A. Melan

University of Pittsburgh
Medical Center,
USA

We used GenScript's plasmid preparation service to prepare large quantities of plasmids that contain our Southern blot probe fragments. This saved our lab considerable time and expense. From the initial order to service delivery, GenScript provided our lab with timely follow-ups and valuable advice. We now have an adequate supply of these probes with no need to grow bacterial cultures every time a new probe is required. Thank you, GenScript!

Customer Reviews

17 Customer Reviews

4.5 out of 5 stars

REQUEST A QUOTE