Custom Polyclonal Antibody Production
GenScript offers a full suite of high quality custom polyclonal antibody production services designed with your needs in mind. Since 2004, our team has produced over 15,000 high quality custom polyclonal antibodies from rabbit, mouse, and rat hosts. The PolyExpress™ protein immunization package carries industry-leading guarantees of WB positive against target protein binding with 1:256,000 ELISA titer. All you need to provide is your target sequence and GenScript gets to work. Read More »
The PolyExpress™ peptide immunization package (SC1180) starts with peptide antigen design using our proprietary OptimumAntigen design tool and finish with affinity purified polyclonal antibodies in just 45 days. The PolyExpress™ protein immunization package (SC1676) starts with protein antigen production and guarantee the delivery of antigen affinity-purified antibody 2 weeks faster than any other vendor.
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Customer pAb Case Study
Learn how polyclonal antibodies maximize your chances of detecting novel target proteins
Key Features of PolyExpress™ Custom Polyclonal Antibody Production Services
- Cost effective and comprehensive: pricing starts from $899 and includes antigen synthesis and purification!
- Fast turnaround time: delivery of purified custom polyclonal antibodies in as little as 45 days.
- Optimal immunization: Our OptimumAntigen design tool and intelligent Antigen Strategy increase specificity and affinity of antibodies.
- Guaranteed results: affinity purified custom polyclonal antibody quantities and ELISA titers of 1:256,000.
- Certified facility: AAALAC International accreditation and OLAW certification, demonstrating our commitment to responsible animal care and use.
Details of PolyExpress™ Custom Polyclonal Antibody Production Services
| Feature | Details | SC1180 | SC1676 |
|---|---|---|---|
| Immunization | Antigen Synthesized | 1 peptide | 1 protein |
| No. of rabbits immunized | 2 | ||
| Deliverables | Negative Control | Total IgG (optional: Pre-immune serum or total serum protein delivery) | |
| Antigen Sample | 2 mg of Peptide Antigen | 200 μg Protein | |
| Antigen Affinity Purified pAb(s) | 1 (2-6 mg total) | 2 (3-12 mg total) | |
| Guarantee | Application | ELISA titer 1:256,000 | WB positive for target protein antigen binding; ELISA Titer 1:256,000 |
| Antibody Yield | 1 purified pAb ≥ 2 mg | 2 separately purified pAbs (1.5 – 6mg/ rabbit) | |
| Timeline | 45 days | 10-11 weeks | |
| Price | From $899 | From $2,599 | |
*GenScript provides peptide synthesis, peptide design, carrier conjugation and antibody purification for free.
**During the development of the antibody, if there is the death of the experimental animal, we will choose the adjusted immunization process for re-immunization to ensure the high quality and efficiency of the order.
| Assays | Western Blot | Sandwich ELISA | IF, IHC, Flow Cytometry |
IP | ChIP |
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Customer case study
Western blot analyses were performed to measure the effect of the Osi21 functional loss on the degradation of endocytosed rhodopsin.
The polyclonal rabbit antiarrestin2 antibody is diluted 1:30,000. (GenScript, NY)
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Experimental Data
Antibodies produced using PolyExpress™ have ELISA titers as high as polyclonal antibodies produced using a traditional protocol.
ELISA analysis of purified antibody generated by either PolyExpress™ protocol or traditional protocol.
ELISA analysis of purified antibody generated by PolyExpress™ Antibody Service.
PolyExpress™ generated an antibody with ELISA titer> 1: 256,000 which is higher than guaranteed.
Polyclonal Antibody Services Recommendations
For polyclonal antibodies targeting poorly defined epitopes we recommend protein antigens. Protein antigens provide the immune system the complete profile of exposed epitopes in the same conformational context the antibody will ultimately recognize the antigen such as FACS, sandwich ELISA, or receptor blocking for example.
Custom Polyclonal Antibody Generation Related Services
Not looking for polyclonal antibodies? We have offerings for every antibody project. Just a few include:
- DNA immunization: a powerful tool for challenging targets such as membrane proteins, toxic and unstable antigens.
- Accelerated mAb Production Service high quality, purified monoclonal antibodies guaranteed to work in your application.
- Semi-custom Monoclonal Antibody Production Services: you can go from antigen sequence to antibody production with as little as 3 easy decisions.
Quotation and Ordering
Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.
