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Custom Polyclonal Antibody Production

Now Featuring A Free Tag!

Promotion Details:

• Free tag promotion can only be added to PolyExpress orders using catalog numbers SC1676, SC1809, SC1180, SC1648, SC1649
• The Tag includes Biotin, HRP, and FITC using catalog numbers SC1061, SC1062, SC1792
• Promotion is only for 0.2mg of tagged pAb
• Promotion runs from 10/01/2018-12/31/2018

GenScript offers a full suite of high quality custom polyclonal antibody production services designed with your needs in mind. Since 2004, our team has produced over 15,000 high quality custom polyclonal antibodies from rabbit, mouse, and rat hosts. The PolyExpress™ protein immunization packages carry industry leading guarantees of WB and IP positive against target protein binding with 1:128,000 ELISA titer. All you need to provide is your target sequence and GenScript gets to work. Read More »

Key Features of PolyExpress™ Custom Polyclonal Antibody Production Services

• Cost effective and comprehensive: pricing starts from $649 and includes antigen synthesis and purification!
• Fast turnaround time: delivery of purified custom polyclonal antibodies in as little as 45 days.
• Optimal immunization: Our OptimumAntigen™ design tool and intelligent Antigen Strategy increase specificity and affinity of antibodies.
• Guaranteed results: affinity purified custom polyclonal antibody quantities and ELISA titers of 1:128,000.
• Certified facility: AAALAC International accreditation and OLAW certification, demonstrating our commitment to responsible animal care and use.

Packages

PolyExpress™ Custom Polyclonal Antibody Production Packages

Protein antigen

PolyExpress™ Premium PolyExpress™ Premium Plus Use recombinant protein as antigen** 2 affinity-purified pAb (1.5-6 mg each) and 200 ug protein antigen 1 affinity-purified pAb (≥3 mg) and 1mg protein antigen Guaranteed to perform in antigen ELISA and WB applications Guaranteed to perform in antigen ELISA, WB, and IP applications

Peptide antigen

PolyExpress™ Basic PolyExpress™ Silver PolyExpress™ Gold Uses 1 peptide to make antibody Uses 2 peptides to make antibodies Uses 3 peptides to make antibodies 1 Ab generated against 1 epitope 2 Abs generated against 2 individual epitopes 3 Abs generated against 3 individual epitopes Guaranteed ≥2 mg purified antibody 2 affinity-purified antibodies (≥2 mg) 3 affinity-purified antibodies (≥2 mg) Application Success Rate

* Silver and Gold packages only apply to a single target protein sequence. Cannot be combined from different protein targets.
**Protein may be full length or a protein fragment ≥ 10 KD depending on which is more likely to generate a successful antibody.
***During the development of the antibody, if there is the death of the experimental animal, we will choose the adjusted immunization process for re-immunization to ensure the high quality and efficiency of the order.

Specifications

Details of PolyExpress™ Custom Polyclonal Antibody Production Services

Feature	Details	Basic (SC1180)	Silver (SC1648)	Gold (SC1649)	Premium (SC1676)	Premium Plus (SC1809)
Immunization	Antigen(s) Synthesized	1 Peptide	2 Peptides	3 Peptides	1 Protein
	Rabbits Immunized	2	4 (2 per peptide)	6 (2 per peptide)	2
Deliverables	Pre-immune Serum	0.5 ml
	Antigen Sample	2 mg of Each Peptide Antigen			200 µg Protein	1 mg protein
	Antigen Affinity Purified pAb(s)	1 (2-6 mg total)	2 (4-12 mg total)	3 (6-18 mg total)	2 (Total 3-12 mg)	1 (Total 3-12 mg)
Guarantee	Application	ELISA Titer 1:128,000			WB (Premium) or WB & IP (Premium Plus) Positive for target protein antigen binding ELISA titer 1:128,000
	Antibody Yields	1 purified pAb (≥2 mg)	2 purified pAbs (≥2 mg)	3 purified pAbs (≥2 mg)	2 purified pAbs (≥3 mg)	1 purified pAb (≥3 mg)
	Timeline	45 Days			10-11 Weeks
Price	Base Package	Starting from $649			Starting from $1,999

*Each package only applies to a single protein sequence
**GenScript provides peptide synthesis, peptide design, carrier conjugation and antibody purification for free
***During the development of the antibody, if there is the death of the experimental animal, we will choose the adjusted immunization process for re-immunization to ensure the high quality and efficiency of the order.

Application

Assays	Western Blot	Sandwich ELISA	IF, IHC, Flow Cytometry	IP	ChIP
Requirements&Rating Rationale	Average affinity and specific recognition.	High specificity and sensitivity. Ability to use same pAb as both capture and detection.	High specificity and sensitivity Conformational epitope recognition	Recognize multiple epitopes recovers more target overcomes obscured epitopes Conformational epitope recognition
Premium Plus
Premium
Gold
Silver
Basic

Selection

Citations and Experimental Data

Customer case study

Western blot analyses were performed to measure the effect of the Osi21 functional loss on the degradation of endocytosed rhodopsin.

The polyclonal rabbit antiarrestin2 antibody is diluted 1:30,000. (GenScript, NY)

J Lee, M Song, S Hong. Negative Regulation of the Novel norpAP24 Suppressor, diehard4, in the Endo-lysosomal Trafficking Underlies Photoreceptor Cell Degeneration. PLoS Genet. June 2013, 9, e1003559

See Full List of Citations »

Experimental Data

Antibodies produced using PolyExpress™ have ELISA titers as high as polyclonal antibodies produced using a traditional protocol.

ELISA analysis of purified antibody generated by either PolyExpress™ protocol or traditional protocol.

ELISA analysis of purified antibody generated by PolyExpress™ Antibody Service.
PolyExpress™ generated an antibody with ELISA titer> 1: 256,000 which is higher than guaranteed.

Polyclonal Antibody Services Recommendations

For polyclonal antibodies targeting poorly defined epitopes we recommend protein antigens. Protein antigens provide the immune system the complete profile of exposed epitopes in the same conformational context the antibody will ultimately recognize the antigen such as FACS, sandwich ELISA, or receptor blocking for example. If financial constraints prevent selection of protein antigen packages, we recommend using the maximum amount of peptide antigens with our Gold package. Read More »

Capitalizing on our time-tested experience and proprietary technologies, GenScript offers guaranteed custom polyclonal antibody services with proven success rates.

	Our OptimumAntigen Design Tool combines the industry's most advanced algorithms with GenScript's professional expertise resulting in: Strong Antigenicity Avoidance of non-surface epitopes Specify desirable cross-reactivity Elimination of re-synthesis delays with a built-in peptide tutorial for synthesis and solubility Choose the best antigen conjugation and presentation options for your desired assay(s) A guaranteed immune response
	Our PolyExpress Gold package increases success rate by using more than one antigen peptide for each protein
	A proprietary immunization adjuvant elicits a stronger and faster cellular immune response with longer immune sustainability, making the production of high-titer, high affinity custom antibodies possible. *High Immunoaffinity   *Increase response   *Diminished toxicity

Custom Polyclonal Antibody Generation Related Services

Not looking for polyclonal antibodies? We have offerings for every antibody project. Just a few include:

• DNA immunization: a powerful tool for challenging targets such as membrane proteins, toxic and unstable antigens.
• MonoExpress™ mAb Production Service high quality, purified monoclonal antibodies guaranteed to work in your application.
• Semi-custom Monoclonal Antibody Production Services: you can go from antigen sequence to antibody production with as little as 3 easy decisions.

Unsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.

Quotation and Ordering

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