×

Custom Polyclonal Antibody Services (Rat)

polyclonal antibody services quotation

These services generate custom rat polyclonal antibodies using peptide antigens designed and optimized using our OptimunAntigen™ Design Tool. Both rat antibody packages come with a guaranteed ELISA titer of ≥1:32,000.

Peptide Supplied Polyclonal Antibody Package (Rat) (Cat. No.: SC1313)

Includes:
Delivery:
Time Frame:
1) Peptide synthesis: (<15 a.a,>85% purity, 7 mg) -50-100 μl of pre-immune serum from each rat2-3 weeks
2) Conjugation: A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH. -ELISA results 1 week
3) Immunization: Three rats, pre-immune bleed, four immunizations/rat -5 ml of antisera from all rats 10 weeks
-5 mg peptide, MS and HPLC reports
4) Shipping1-2 weeks
Total 16 weeks

Complete Protein G-Purified Peptide Polyclonal
Antibody Package (Rat) (Cat. No.: SC1314)

polyclonal antibody services quotation
Includes:
Delivery:
Time Frame:
1) Peptide synthesis: (<15 a.a, >85% purity, 7 mg) -50-100μl pre-immune serum 2-3 weeks
2) Conjugation: A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH. -ELISA results 1 week
3) Immunization: Three rats, pre-immune bleed, four immunizations/rat -5 mg peptide, MS and HPLC reports 10 weeks
4) Antibody purification by protein G column -10-25 mg purified antibody from all antisera 1 week
5) ELISA test
4) Shipping1-2 weeks
Total 17 weeks

Standard Protocol (Three animals)

Steps Time from Project Initiation Time from Immunization Activity Description
Peptide synthesis ≈2 weeks
Day-14
-
Peptide conjugation to carrier ≈3 weeks
Day-7
-
Control serum collection ≈4 weeks Day -1
Pre-bleed (≈0.1 ml serum)
Primary immunization ≈4 weeks Day 0
50 µg per rat peptide-KLH conjugate, -Freunds's Complete Adjuvant, Intraperitoneal injection
1st boost ≈6 weeks Day 14
25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection
Test bleed and ELISA ≈7 weeks Day 21
0.01-0.02 ml serum per rat. ELISA and initial response evaluation.
2nd boost ≈9 weeks Day 35
25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection
Test bleed and ELISA ≈10 weeks Day 42
0.01-0.02 ml serum per rat. ELISA and initial response evaluation.
3rd boost ≈12 weeks Day 56
25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection
Production bleed ≈13 weeks Day 63
1.5 – 2 ml serum per rat
ELISA and shipping ≈15 weeks Day 77
Extend or terminate

Note:

If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.

Quotations and Ordering

Ordering Process
polyclonal antibody services quotation
  • Request a quote and our professional account managers will contact you within 24 hours
  • If you need to submit your own physical antigen, mail it with the antigen submission form to: Antibody Services, GenScript, 860 Centennial Ave., Piscataway, NJ 08854, US.

Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.

 
*
*
*
*