X Enzyme for GCT
Project Goal: Deliver work cell bank for this GCT enzyme
Comment: The clone with the highest expression level was selected to
construct MCB and WCB
1. Expression Evaluation & Restriction Map
Result: MCB and WCB were confirmed by expression level evaluation and
restriction map of plasmid
2. Phage Test
Use Double-Layer Agar (DLA) technique to test phage of master cell
bank and working cell bank.
Result: No phage contamination in the master cell bank and working cell bank.
3. Plasmid Stability Test
Passage the WCB cells until P20 with antibiotics and test the
Result: The expression level of the working cell bank remained stable and did
not show a significant reduction from the SDS-PAGE result of P1, P5, P10, P15 and P20.
Passage of the WCB cells until P20 without antibiotics to test
Result: There was no obvious plasmid loss in the passages of the work cell bank
(P1, P5 and P10).
4. Long-Term Viability Test
The viability and expression level of the working cell banks are
tested to determine the storage time.
||Check point of storage time (Months)