DNA Sequencing FAQs
- I have template need to sequence with multiple primers, how do I place the order and prepare the samples? Can I put the primer together?
A: One Sanger Sequencing reaction can only be sequenced with one template and with one primer. The same template sequenced with multiple primers is considered as multiple reactions. On the order form, please input each reaction as a separate item. For sample submission, please put the primers into separate tubes.
- I try to submit my order form, but it is not going through.
A: There may be some error with your order form. For the sample submission form, please only fill or select the information but not insert/delete/modify any of the cells and format that already in the blank order form. For example, do not type “premix” for primer type, instead you should choose from the list "Premixed". The information for sample type, primer type and primer name (when using standard primer) must be chosen from the list, so it will match the system.
- Can I submit handwritten sample submission forms with my samples? Can I modify my sequencing request by handwriting on the submission form?
A: Handwritten submission forms or any type of handwriting information is NOT acceptable, GenScript will only use the information in the system to process your sequencing order. Please placing the order on our website, or email the sample submission form to firstname.lastname@example.org if you have any issue with the online ordering.
- Can I submit my samples with plate but no a full plate?
A: Yes, we do accept non-full plate sample submission. However, please arrange your samples vertically (in columns) and leave no empty wells in between. For example, if you have 48 samples, it must be filled in well A1, B1….to H6.
- I don’t have enough samples and couldn’t get the required concentration, can I submit with lower concentration and volume than requested?
A:Low concentration samples may give poor quality sequencing data, and low volume samples may not be eligible for a repeat sequencing attempt.
- Can GenScript synthesis sequencing primers for me? Can I get the sequencing primers ship to me? Will GenScript store my sequencing primers for my future orders?
A:GenScript provides sequencing primer synthesis service with extra charge. The synthesized sequencing primers are for onsite sequencing service use only, Sequencing department will not ship back any samples or primers. The synthesized sequencing primer will be kept with the corresponding sequencing order on site for 1-2 weeks for repeat sequencing purposes only.
- Will GenScript send my samples back if I need? Will GenScript keep my samples for future orders?
- I have shipped my samples few days ago, but I am still not getting any data back.
- Where can I find my sequencing data?
- Why do I receive longer sequence read outs than my samples should have? Why does the readout not match my samples?
- Why are my sequencing reads so short?
- The sequencing data is not in good quality, will GenScript re-run them? Do I need to send more samples for the re-run?
A:GenScript does not send sequencing samples back to customers. The sequencing samples will be kept on site for 1-2 weeks for repeat sequencing purposes only. If you want to use the same samples for different orders, a new sample submission is required.
A: Please check with your courier (FedEx, UPS, USPS) and make sure your shipments have arrived at the correct address, with recipient name DNA Sequencing Service.
GenScript USA Inc.
DNA Sequencing Service
860 Centennial Avenue
Piscataway, NJ 08854, U.S.
If you did not enclose the GenScript order ID in your package, please expect the delays or someone will contact you to identify & match the contents of your package with your account.
If you submit samples by following the guidelines and the packages were delivered, please have your order ID and the shipment tracking number send to us to check the status.
A:Log-in to our website https://www.genscript.com/account/login.html In the side bar, click "My Account". On the next page, click "My Sequencing". On the next page, search for your order ID.In the side bar, click 'My Account". On the next page, click "My Sequencing". On the next page, search for your order ID.
A: Sequence readout from .seq files may include low-confidence reads. You may need to check the .ab1 files for the QV scores of the peaks, and eliminate the false readings.
A: Our Sanger sequencing guarantees clean reads up to 500-750 base pairs depending on multiple circumstances. If you have clean reads for the first several hundred base pairs before a sudden/early drop in base read confidence, your template sequence may have high G-C content (60+%), repeated base regions, or secondary structures (hairpins, poly A-T, etc.) These characteristics make a template difficult to sequence through, and will require one of our alternative protocols.
If you know in advance that your template sequence contains these difficult regions (extremely High GC content, Poly structure, Hairpin, Long repeated regions), you can request our alternative protocol "Secondary structure" under the "Special Requests" column in the order form. Extra charge for "Secondary structure" may apply.
A: Details for how to request repeat sequencing are included in the same automatic email notifying you when your sequencing data is uploaded.
Each individual reaction is eligible for one free repeat attempt, and GenScript will review your data and adjust protocol as needed for better results. The repeated data will also upload to your account.
GenScript do not accept new samples submission or change of the primer and special request for free repeat, by following the submission guideline, the sample amount will be enough for one round repeat.