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The primer extension assay to detect the blocking activity of Taq Antibody.
To test the blocking activity of Hot Start Taq antibody, a primer extension assay was done as follows. A pair of primers was designed with a 14 bp overlap. One is 24 bp and the other one is 41 bp. The primers were annealed and incubated with Taq DNA polymerase (lane 1-2) or Hot Start Taq DNA polymerase (lane 3-4) for 0.5 h at 65℃. The extension result was separated on an Urea PAGE gel. No primer dimers were observed with Hot Start Taq polymerase.
The PCR specificity detection of Hot Start Taq DNA polymerase.
To test the specificity of amplification, a 500 bp long HPRT fragment was amplified from human genomic DNA using Hot Start Taq DNA polymerase (lanes 1-2) and Taq DNA polymerase (lane 3-4). No non-specific bands were observed in the amplification reaction with the Hot Start Taq DNA polymerase.
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