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Catalog Products » Recombinant Proteins » Enterokinase (EK), His, Lyophilized, Bovine

Enterokinase (EK), His, Lyophilized, Bovine

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys(DDDDK↑). Enterokinase will not work if the recognition site is followed by a proline. rbEKhas the highest activity than EK of other species and is used wildly in biochemical applications. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.
Recombinant Bovine Enterokinase (His-tagged) (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids, 6 × His at C-terminus. A fully biologically active molecule, rbEK has a molecular mass of 40 kDa and is obtained by proprietary chromatographic techniques at GenScript.

Enteropeptidase, ENTK, PRSS7

For research use only

Biological Activity 100 IU/μg
Source P. pastoris
Species Bovine

Purity > 95% as analyzed by SDS-PAGE.
Endotoxin Level <0.2 EU/μg, determined by LAL method.
Reconstitution Reconstitute lyophilized EK powder in sterile deionized water at 1mg/ml, then dilute in EK Dilution/Storage Buffer according to your requirement.
Formulation Lyophilized from a 0.2 μm filtered solution in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2.
Measured Molecular Weight 40 kDa, observed by reducing SDS-PAGE.
Storage Lyophilized recombinant Bovine Enterokinase (His-tagged) (rbEK) remains stable up to one year at -20°C from date of receipt. Please avoid freeze-thaw cycles.

Cat. No. Product Name Quantity Price
Z03004-100
Enterokinase, His, Bovine - 100 IU
$55.00
 


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Cat. No. Z03376
Size Price
10 μg $65.00
50 μg $95.00
1 mg $700.00