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GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free

*This product has been discontinued!*
GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free is a mutant form of Cas9 nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with an N-terminal nuclear localization signal (N-NLS). GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free delivers higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Z03692
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Description

The GenCRISPR™ Ultra eSpCas9 product line provides customers with a selection of research use, basic GMP and GMP compliant Cas9 nucleases. The Cas9 protein can be formed with the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex to perform gene editing has been shown to reduce the challenges encountered with other CRISPR gene editing techniques such as viral and plasmid delivery. Challenges include off-target effects, cell viability and transcription/translational challenges.

GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free is a mutant form of Cas9 nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with an N-terminal nuclear localization signal (N-NLS). GenCRISPR™ Ultra eSpCas9-N-NLS Research, tag-free delivers higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Overview
Key Features High knockout efficiencies: Consistent high performance in in-vitro plasmid cleavage test.
Tag-free: No extra tag amino acid
DNA-free: No external DNA added to the system.
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Applications
gRNA-dependent double-stranded DNA cleavage
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Properties
Expression System Recombinant eSpCas9 with an N-terminal NLS expressed by E.coli
Species S. pyogenes
Tag Tag-free
Molecular Weight ~160 kDa
Concentration 10 mg/ml
Active temperature This eSpCas9 is active at 37°C.
Formulation Supplied as a solution of 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol pH 8.0 at 25°C. 
Storage & Stability This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Quality Control Specifications
AssaySpecifications
AppearanceClear, colorless liquid
Purity≥ 95% as analyzed by SDS-PAGE
≥ 90% as analyzed by SEC-HPLC
Concentration by A28010 mg/ml±1 mg/ml
Bioactivity ( in vitro)≥ 90%
Residual DNaseNon-specific DNase activity
Residual RNaseNon-specific RNase activity
Endotoxin Level≤ 10 EU/mg as analyzed by gel clotting method
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