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GenScript ExpressPlus™ PAGE Gels have been upgraded to a 10×8 and 10×10 series compatible with more gel tanks. More researchers can experience precast gels with a shorter running time, larger loading volume, higher transfer efficiency at affordable prices. The ExpressPlus™ PAGE Gels are cast in a weak acidic pH environment that minimizes the hydrolysis of polyacrylamide and results in extra gel stability and superior band resolution.
* Price valid on GenScript ExpressPlus? PAGE Gel orders for the US and Canada only. Customers ordering from other countries please contact Product@GenScript.com for the latest pricing information in your region.
** GenScript ExpressPlus™ PAGE Gels are formulated using 1x Bis-Tris buffer (pH 6.8). For best results, please use GenScript running buffer (Cat. No. M00138) for gel running, and GenScript transfer buffer (Cat. No. M00139) for protein transfer after running the ExpressPlus™ PAGE gels. Otherwise, please prepare buffers according to our protocol.
The Protein Migration Table below helps you choosing the appropriate gels for protein electrophoresis. The data of this table is built on electrophoresis at 10 volts for 55 min using ExpressPlus™ PAGE gels.
Recipe of 10X MOPS Buffer:
Recipe of 10X MES Buffer
Recipe of 5X Sample Buffer
Recipe of 1X Protein Sample Buffer
Compatible Gel Tanks:
Lane 1, 2: GenScript Broad Protein maker Ⅱ
Lane 3, 4: E.Coli lysate
Lane 5: TaKaRa Protein Molecular Weight Marker (Broad) Code NO. 3452
Lane 6, 7, 8, 9, 10: GenScript PAGE-MASTER Protein Standard (M00516), each sample of lane 6, 7, 8, 9 and 10 contains: 10 μl, 5 μl, 2.5 μl, 1.25 μl, 0.63 μl.
Lane 11, 12: GenScript PAGE-MASTER Protein Standard Plus (MM1397-500)
EasyWestern Protein Standard (GenScript, MM0908), was separated on a 4-20% ExpressPlus™ PAGE Gel and transferred to nitrocellulose membrane. The blot was blocked overnight with 10% fat-free milk, detected with a Anti-RABBIT IgG (H&L) (GOAT) Antibody (Rockland, 611-132-122). Scanned by odyssey(LI-COR). Lane 1-5 contained Protein G at 200ng, 100ng, 50ng, 10ng and 5ng, Lane 6-10 contained EasyWestern Protein Standard at 4μl, 2μl, 1μl, 0.5μl, 0.25μl, respectively.
Lane 1, 2, 6, 7, 11, 12: 6 μl E.Coli lysate
Lane 3, 8: 5 μl NEB Protein Standard (P7703S).
Lane 4, 9: 5 μl GenScript PAGE-Master Protein
Lane 5, 10: Life Tech. Mark12™ Unstained
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