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Broad sgRNA Database
Genome-wide gRNA databases for CRISPR genome editing and transcription activation
To facilitate your research using CRISPR technology, GenScript provides genome-wide databases containing pre-validated gRNA sequences design by the Broad Institute of Harvard and MIT. If you want to view all, please click gRNA list.
Reference: Sanjana et al., Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
- Ready for delivery: More than 20,000 gRNA constructs are in-stock and ready for delivery at $99/clone.
- Full-Genome Coverage:
- GenScript\'s gRNA Database contains 6 unique, pre-validated gRNA sequences targeting each of the 19,050 genes in the human genome and 20,611 genes in the mouse genome. SpCas9 gRNA sequences are targeted to constitutive exons and designed for minimal off-target effects.
- GenScript\'s SAM Database contains 6 unique, pre-validated gRNA sequences targeting each of the 19,050 genes in the human genome. SAM gRNA sequences target the first 200bp upstream of each transcription start site.
CRISPR Products and Services at GenScript
New! CRISPR crRNA/Cas9 Protein Reagents
Customizable, DNA-free, ready-for-transfection crRNA/Cas9 particles.
| CRISPR Plasmids
DNA plasmids for single guide RNA and/or Cas9 expression |
Cell Line Gene Editing
Validated knock-out cell line service using CRISPR technology. |
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| CRISPR Libraries
Genome-wide or pathway-specific CRISPR knock-out or activation libraries for screening experiments. |
Microbial Gene Editing
Validated knock-out and knock-in mutagenesis in bacteria and yeast. |
