Gulo CRISPR guide RNA, gulonolactone (L-) oxidase CRISPR guide RNA[house mouse]

CRISPR gRNA for genome editing with WT SpCas9 vector or cas9 protein

The following gRNA sequences were designed by Feng Zhang’s laboratory at the Broad institute* to uniquely target the Gulo gene within the house mouse genome. These gRNA sequences are for use with WT SpCas9, or as crRNA for use with WT SpCas9 protein, to introduce a DSB for genome editing. These sgRNA sequences were validated in Sanjana N.E., Shalem O., Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4.

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