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Want to learn more about GenScript GenBuilder™ DNA Assembly?
Table of Contents
Introduction (2)
SECTION 1. GenBuilder™ DNA Assembly (3)
- Experiment 1. One-step construction of the five-gene Violacein biosynthetic pathway (3)
- Experiment 2. Testing comparative efficiency of cloning one to six gene fragments into pUC57 (4)
- Experiment 3. One-step cloning of 1 kb – 8 kb fragments into pUC57 (5)
- Experiment 4. Determining cloning efficiency using 15 bp – 40 bp homologous arms and varying DNA concentrations (5)
- Experiment 5. Determining the effect of insert size vs. number of fragments on cloning efficiency (7)
- Experiment 6. Comparing cloning efficiency between 15 and 60 minutes assembly reactions (8)
- Experiment 7. Determining cloning efficiency using unpurified PCR products (9)
- Experiment 8. Using ssDNA as a bridge to for site directed mutagenesis (10)
- Experiment 9. Using ssDNA as a bridge to create saturation mutagenesis for directed evolution (11)
- Experiment 10. Comparing GenBuilder™ DNA Assembly with other DNA Assembly systems (13)
SECTION 2: GenBuilder™ Plus DNA Assembly (16)
- Comparison between GenBuilder™ and GenBuilder™ Plus Cloning kits (16)
- Experiment 1. Testing cloning efficiency with different DNA concentrations (16)
- Experiment 2. Testing cloning efficiency with different lengths of DNA overlap (18)
- Experiment 3. Testing cloning efficiency with different number of fragments (20)
- Experiment 4. Testing cloning efficiency with ssDNA assembly (21)
- Experiment 5. Comparing GenBuilder™ Plus DNA Assembly with other DNA Assembly systems (22)
- 5.1. Comparing DNA Assembly performance of 5 fragments with different DNA concentrations (22)
- 5.2. Comparing DNA Assembly performance of 5 fragments with different DNA overlaps (23)
- 5.3. Comparing DNA Assembly performance of different number of DNA fragments (24)
